Insect viruses : biotechnological applications / edited by Bryony C. Bonning.

Format:

Book

Contributor:

Bonning, Bryony C.

Series:

Advances in virus research ; 68.

Advances in virus research ; 68

Language:

English

Subjects (All):

Insects--Viruses.

Insects.

Viruses.

Physical Description:

1 online resource (512 p.)

xiv, 532 p. il. 8algunas en color) 24 cm

Place of Publication:

San Diego : Academic Press, 2006.

Language Note:

English

Summary:

Baculoviruses are perhaps unique among viruses in the breadth of their biotechnological applications: these insect specific viruses are used not only for insect pest management purposes, but also as laboratory research tools for production of recombinant proteins and for protein display, and as potential vectors for human gene therapy. In addition to highlighting recent advances, this volume provides a comprehensive review of the biotechnological applications of these and other insect viruses in both the academic and private sectors.

Contents:

Cover Page; Contents; Chapter 1: Milestones Leading to the Genetic Engineering of Baculoviruses as Expression Vector Systems and Viral Pesticides; I. Introduction; II. Baculovirus Advances Before the Application of Recombinant DNA; A. Virus Structure, Composition, and Infectivity; B. In Vivo and In Vitro Developments for Virus Infection and Replication Studies, Preliminary Studies of Protein and Nucleic Acid Composition; C. Insect Cell Culture Advances; Budded Virus (BV) and Occlusion-Derived Virus (ODV) Structure and Role in Infection; III. Recombinant DNA Technologies

A. Virus Identification, Genotypic Variation, Physical Mapping ofGenomesB. Functional Mapping, Gene Identification, Virus Protein Structure and Function, Regulation of Viral Gene Expression; C. Search for the Polyhedrin Gene and Development of the BEVS; D. BEVS: Strategies for Optimizing Recombinant Protein Expression, Expression of Multiple Recombinant Proteins, Enhancement of Foreign Gene Expression; IV. Quality Improvements: Expression Vectors, Enhanced Expression, Secretion, and Protein Integrity; V. Pathways of Baculovirus Invasion and Infection

VI. Genetically Engineered Viral PesticidesA. Development of Genetically Engineered Baculovirus Pesticides; VII. Other Notable Developments Emerging from Baculovirus Molecular Biology and the BEVS; A. Stable Transformation of Insect Cell Lines and Insects; B. Baculovirus-Mediated Gene Delivery in Mammalian Cells; VIII. Conclusions; References; Chapter 2: Polydnavirus Genes that Enhance the Baculovirus Expression Vector System; I. The BEVS: Advantages and Limitations; II. Enhancement of the BEVS in Insect Cells: General; A. Addition of Various Gene Elements to the Virus

B. Modification of Secretion and Posttranslational ProcessingC. Improvement of Protein Integrity; III. Enhancement of the BEVS in Insect Cells by Campoletis sonorensis Ichnovirus Vankyrin Proteins (Vankyrin-Enhanced BEVS).; A. CsIV Vankyrin Gene Family; B. Enhancement of BEVS by Coexpressing a Vankyrin Protein from a Dual Expression Vector; C. Expression of Conventional BEVS Is Enhanced in a Transformed Cell Line Expressing Vankyrin Genes; D. Summary and Further Application; Acknowledgments; References

Chapter 3: Baculovirus Display: A Multifunctional Technology for Gene Delivery and Eukaryotic Library DevelopmentI. Introduction; II. Targeting of Baculoviral Vectors by Surface Display; III. Altering the Tropism of Baculoviral Vectors Through Pseudotyping; IV. Baculovirus Display of Immunogens; V. Generation of Display Libraries; VI. Summary; References; Chapter 4: Stably Transformed Insect Cell Lines: Tools for Expression of Secreted and Membrane-anchored Proteins and High-throughput Screening Platforms for Drug and Insecticide Discovery; I. Introduction

II. Generation of Stably Transformed Cell Lines

Notes:

Description based upon print version of record.

Includes bibliographical references.

ISBN:

1-280-63667-X

9786610636679

0-08-046478-5

OCLC:

476019978