Membrane protein crystallization / edited by Larry DeLucas.

Format:

Book

Contributor:

DeLucas, Lawrence J.

Series:

Current topics in membranes ; v. 63.

Current topics in membranes, 1063-5823 ; v. 63

Language:

English

Subjects (All):

Membrane proteins.

Crystallization.

Physical Description:

1 online resource (334 p.)

Place of Publication:

Amsterdam ; London : Academic Press, 2009.

Language Note:

English

Summary:

This volume of Current Topics in Membranes focuses on Membrane Protein Crystallization, beginning with a review of past successes and general trends, then further discussing challenges of mebranes protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This publication also includes tools to enchance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes, establishing Membrane Protein Crystallization as a needed, practical reference for researche

Contents:

Front Cover; Membrane Protein Crystallization; Copyright Page; Dedication Page; Contents; Contributors; Preface; Previous Volumes in Series; Introduction; Chapter 1: Introduction to the Crystallization of Biological Macromolecules; I. Overview; II. Introduction; III. The Requirement for Supersaturation; References; Chapter 2: In Vitro Synthesis of Posttranslationally Modified Membrane Proteins; I. Overview; II. Introduction; III. Materials and Methods; IV. Results; V. Conclusions; Acknowledgments; References; Chapter 3: Harnessing Photosynthetic Bacteria for Membrane Protein Production

I. OverviewII. Introduction; III. Foreign Gene Expression in Rhodobacter sphaeroides; IV. Membrane Protein Preparations for Structural and Functional Studies; V. Practical Aspects of Heterologous Protein Expression in Rhodobacter; Concluding Statements; Acknowledgments; References; Chapter 4: Monoacylglycerols: The Workhorse Lipids for Crystallizing Membrane Proteins in Mesophases; I. Overview; Introduction; III. In Meso Crystallogenesis in Molecular Detail; VI. Lipid Synthesis and Purification; VII. Conclusions; Acknowledgments; References

Chapter 5: Practical Aspects of Membrane Proteins Crystallization in BicellesI. Overview; II. Introduction; III. Bicelles; IV. Phase Behavior of Bicelles; VI. Crystal Packing; VII. Practical Information; VIII. Conclusion; References; Chapter 6: Membrane Protein Crystallization: Approaching the Problem and Understanding the Solutions; I. Overview; II. Introduction; IV. Vapor Diffusion; V. Microdialysis; VI. Microfluidics; VII. Counter-Diffusion; VIII. Novel Methods using Lipids in Crystallization; IX. How to obtain Crystals from a Membrane Protein of Choice; X. Optimization of Initial ""Hits""

XI. SeedingXII. Is it Possible to Preselect Crystallization Conditions That are Inherently More Favorable for Crystallization?; XIII. Conclusions; Acknowledgments; References; Chapter 7: Tools to Enhance Membrane Protein Crystallization; I. Overview; II. Introduction; Self-Interaction Chromatography; V. Validation Studies: SIC Accurately Quantifies B; References; Chapter 8: Advances in Microfluidic Membrane Protein Crystallization Techniques; I. Overview; II. Membrane Protein Crystallization; III. Microfluidics; IV. Crystallization by FID in Polydimethylsiloxane (PDMS) Devices

V. Membrane Protein Crystallization in Gradients Established in MicrochannelsVI. Plug-Based Membrane Protein Crystallization in Microcapillaries; VII. Conclusion; Acknowledgments; References; Chapter 9: Crystallization of Photosynthetic Membrane Proteins; I. Overview; II. Overview of Crystallization of Photosynthetic Membrane Proteins; III. Biological Parameters that Influence Crystallization; IV. Physical-Chemical Parameters that Influence Crystallization; V. Crystallization Techniques; VI. Determination of Phase Diagrams; VII. Seeding Techniques; References

Chapter 10: A Practical Guide to X-Ray Crystallography of beta-barrel Membrane Proteins: Expression, Purification, Detergent Selection, and Crystallization

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-282-25825-7

9786612258251

0-08-096159-2

OCLC:

407023926