The zebrafish : genetics, genomics, and informatics / edited by H. William Detrich, Monte Westerfield, Leonard I. Zon.

Format:

Book

Contributor:

Detrich, H. William, III

Westerfield, Monte.

Zon, Leonard I.

Series:

Methods in cell biology ; v. 77.

Methods in cell biology ; v. 77

Language:

English

Subjects (All):

Zebra danio--Cytology.

Zebra danio.

Zebra danio--Genetics.

Zebra danio--Research--Methodology.

Physical Description:

1 online resource (713 p.)

Edition:

2nd ed.

Place of Publication:

Amsterdam ; London : Elsevier/Academic Press, c2004.

Language Note:

English

Summary:

This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. This second volume covers advances in forward and reverse genetic techniques, provides an update on the zebrafish genome and gene/mutant mapping technologies, examines the new systems for efficient transgenesis in the zebrafish, provides an in-depth view of informatics and the emerging field of comparative genomics, and considers the extensive infrastructure now available to

Contents:

Front Cover; The Zebrafish: Genetics, Genomics, and Informatics; Copyright Page; CONTENTS; Contributors; Preface; Dedication; PART I: Forward and Reverse Genetics; Chapter 1. Retroviral-Mediated Insertional Mutagenesis in Zebrafish; I. Introduction; II. Mutagenesis; III. Cloning the Mutated Genes; IV. Future Directions; References; Chapter 2. Genetic Screens for Maternal-Effect Mutations; I. Introduction; II. Strategies for Maternal-Effect Screens; III. Selection of Lines for Genetic Screens; IV. Recovery and Maintenance of Maternal-Effect Mutations; V. Mapping Maternal-Effect Mutations

VI. Solutions, Materials, and ProtocolsVII. Conclusions; References; Chapter 3. Behavioral Screening Assays in Zebrafish; I. Introduction; II. General Considerations; III. Behavioral Assays; IV. Conclusions; References; Chapter 4. Target-Selected Gene Inactivation in Zebrafish; I. Introduction; II. Gene Targeting Strategies; III. Target-Selected Mutagenesis in Zebrafish; IV. Discussion; V. Materials and Methods; References; Chapter 5. A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish; I. Introduction

II. Rationale for Reverse Genetics in ZebrafishIII. Rationale for Using the CEL1 Assay to Detect Induced Mutations; IV. Rationale for Generating a Cryopreserved Mutant Library; V. Method of N-Ethyl-N-Nitrosourea (ENU) Mutagenesis and Rearing of F1 Founder Fish; VI. Generating a Cryopreserved Mutant Library; VII. Isolating Genomic DNA; VIII. Choosing Fragments to Screen; IX. CEL1 Endonuclease Assay; X. Recovery of Mutations from Cryopreserved Sperm; XI. Materials Cost Estimate; References; Chapter 6. Production of Zebrafish Germline Chimeras by Using Cultured Embryonic Stem (ES) Cells

I. IntroductionII. Methods; III. Reagents; IV. Future Directions; References; Chapter 7. Nonconventional Antisense in Zebrafish for Functional Genomics Applications; I. Introduction; II. General Use of Nonconventional Antisense Tools in Zebrafish; III. Use of Morpholinos (MOs) as Antisense Tools in Zebrafish; IV. Comparison of MO-Based Screening Success to Mutational Methods; References; Chapter 8. Downregulation of Gene Expression with Negatively Charged Peptide Nucleic Acids (PNAs) in Zebrafish Embryos; I. Introduction; II. Materials and Methods; III. Results; IV. Discussion; V. Summary

ReferencesChapter 9. Photo-Mediated Gene Activation by Using Caged mRNA in Zebrafish Embryos; I. Introduction; II. Synthesis of Bhc-Caged mRNA; III. Microinjection of the Bhc-Caged mRNA; IV. Uncaging by Illumination of UV Light; V. Titration of Caging Efficiency; VI. Injection of Dimethyl Sulfoxide (DMSO) Solution of Caged mRNA; References; Chapter 10. Current Status of Medaka Genetics and Genomics The Medaka Genome Initiative (MGI); I. Introduction; II. Current Status of Medaka Genetics; III. Current Status of Medaka Genomics; IV. Other Progress

V. Internet Web Sites Relevant to Medaka Resources and Databases

Notes:

Previous ed.: 1998.

Includes bibliographical references and index.

ISBN:

9786611021085

9781281021083

1281021083

9780080522517

0080522513

OCLC:

476073240