Methods in cell biology. Volume 113, Laboratory methods in cell biology : imaging / edited by P. Michael Conn.

Format:

Book

Author/Creator:

Conn, P. Michael.

Contributor:

Conn, P. Michael, editor.

Series:

Methods in cell biology ; v. 113.

Methods in cell biology ; volume 113

Gale eBooks

Language:

English

Subjects (All):

Cell culture--Laboratory manuals.

Cell culture.

Cytology--Technique.

Cytology.

Physical Description:

1 online resource (xv, 414 pages, 16 unnumbered pages of plates) : illustrations (some color).

Place of Publication:

Burlington : Elsevier Science, 2013.

Oxford : Academic Press, 2013.

Language Note:

English

Summary:

Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone. Covers the most important laboratory methods in cell biology<li

Contents:

Front Cover; Series Editors; Laboratory Methods in Cell Biology: Imaging; Copyright; Contents; Contributors; Dedication; Preface: Laboratory Methods in Cell Biology; CHAPTER 1 - STRUCTURAL DYNAMICS OF NUCLEIC ACIDS BY SINGLE-MOLECULE FRET; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL 1-SMFRET IN SEALED SAMPLE CHAMBER; 6 PROTOCOL 2-FLOW CELL FOR SMFRET; Acknowledgements; References; CHAPTER 2 - VISUALIZING THE LOCATION AND THE DYNAMICS OF GENE EXPRESSION IN LIVING ANIMALS THROUGH BIOLUMINESCENCE IMAGING; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References

CHAPTER 3 - QUALITY CONTROL PROTOCOL FOR CONFOCAL SYSTEMS1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 4 - ELECTRON MICROSCOPY OF BONE; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 5 - IMMUNOSTAINING: DETECTION OF SIGNALING PROTEIN LOCATION IN TISSUES, CELLS AND SUBCELLULAR COMPARTMENTS; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 6 - BIMOLECULAR FLUORESCENCE COMPLEMENTATION (BIFC) ANALYSIS OF PROTEIN-PROTEIN INTERACTION: HOW TO CALCULATE SIGNAL-TO-NOISE RATIO; 1 PURPOSE; 2 THEORY

3 EQUIPMENTS4 MATERIALS; 5 PROTOCOL; References; CHAPTER 7 - IMMUNOHISTOCHEMICAL DETECTION OF NEUROPEPTIDES/TRANSMITTERS IN MAMMALIAN BRAIN; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 8 - INTRAVITAL IMAGING OF AUTOREACTIVE T CELLS IN LIVING ANIMALS; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 9 - GENETICALLY ENCODED CLICK CHEMISTRY FOR SINGLE-MOLECULE FRET OF PROTEINS; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; Acknowledgments; References

CHAPTER 10 - HIGH-THROUGHPUT METHODS FOR THE DETECTION OF PROTEIN OVEREXPRESSION USING FLUORESCENCE MARKERS1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 11 - CORRELATIVE MICROSCOPY; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOLS; Acknowledgments; References; CHAPTER 12 - STEREOLOGY OF THE LUNG; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS AND MATERIALS; 4 PROTOCOLS; Acknowledgments; References; CHAPTER 13 - QUANTITATIVE 3D CELL-BASED ASSAY PERFORMED WITH CELLULAR SPHEROIDS AND FLUORESCENCE MICROSCOPY; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS

5 PROTOCOLAcknowledgments; References; CHAPTER 14 - OPTIMIZING FLUORESCENCE EXCITATION AND DETECTION FOR INTRAVITAL TWO-PHOTON MICROSCOPY; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; CHAPTER 15 - TWO-DIMENSIONAL CRYSTALLIZATION BY DIALYSIS FOR STRUCTURAL STUDIES OF MEMBRANE PROTEINS BY THE CRYO-EM METHOD ELECTRON CRYSTALLOGRAPHY; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; CHAPTER 16 - IN SITU HYBRIDIZATION; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References

CHAPTER 17 - RNA IMAGING: TRACKING IN REAL-TIME RNA TRANSPORT IN NEURONS USING MOLECULAR BEACONS AND CONFOCAL MICROSCOPY

Notes:

"ISSN: 0091-679X."

Includes bibliographical references and index.

ISBN:

9781283941587

1283941589

9780124078079

0124078079

OCLC:

881385194