Mitochondria / edited by Liza A. Pon, Eric A. Schon.

Format:

Book

Contributor:

Pon, Liza A.

Schon, Eric A.

Series:

Methods in cell biology ; 80.

Methods in cell biology ; 80

Language:

English

Subjects (All):

Mitochondria.

Cells.

Physical Description:

1 online resource (945 p.)

Edition:

2nd ed.

Place of Publication:

Boston : Academic Press, c2007.

Language Note:

English

Summary:

This book provides an update on the step-by-step ""how to"" methods for the study mitochondrial structure, function, and biogenesis contained in the successful first edition. As in the previous edition, the biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls from each method.

Contents:

Front Cover; Methods in Cell Biology; Copyright Page; Contents; Contributors; Preface; Part I: Isolation and Subfractionation of Mitochondria; Chapter 1: Isolation and Subfractionation of Mitochondria from Animal Cells and Tissue Culture Lines; I. Introduction; II. General Features of Mitochondrial Preparations; A. Cell Rupturing by Mechanical and/or Chemical Means and Resuspension in Isolation Medium; B. Differential Centrifugation; C. Storage; D. Criteria of Purity and Intactness; III. Mitochondria from Beef Heart; A. Small-Scale Preparation

B. Preparation of Coupled Submitochondrial ParticlesC. Broken Submitochondrial Particles; D. Keilin-Hartree Heart Muscle Preparation; E. Cytochrome c-Depleted and Cytochrome c-Reconstituted Mitochondria and ETPH; F. CoQ-Depleted and CoQ-Reconstituted Mitochondria; IV. Mitochondria from Rat Liver; A. Standard Preparation; B. Gradient-Purified Rat Liver Mitochondria; C. Liver SMP; D. Mitochondria from Rat Hepatocytes; E. Isolation of Mitochondria from Frozen Tissues; F. Preparation of Rat Liver Mitoplasts; G. Phospholipid-Enriched Mitoplasts and SMP with or Without Excess CoQ

V. Mitochondria from Skeletal MuscleVI. Synaptic and Nonsynaptic Mitochondria from Different Rat Brain Regions; VII. Mitochondria from Kidney and Testis; VIII. Mitochondria from Hamster Brown Adipose Tissue; IX. Mitochondria from Insect Flight Muscle; X. Mitochondria from Porcine Adrenal Cortex; XI. Mitochondria from Human Platelets; A. Crude Mitochondrial Membranes; B. Coupled Mitochondrial Particles from Platelet Mitochondria; XII. Mitochondria from Fish Liver; XIII. Mitochondria from Sea Urchin Eggs; XIV. Mitochondria and Kinetoplasts from Protozoa; XV. Mitochondria from Fish Erythrocytes

XVI. Mitochondria from Caenorhabditis elegansXVII. Mitochondria from Drosophila; XVIII. Mitochondria and Mitoplasts from Cultured Cells; Acknowledgments; References; Chapter 2: Purification and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae; I. Introduction; II. Isolation of Mitochondria; A. Growth of Yeast Cells; B. Isolation of Crude Mitochondria; C. Purification of Crude Mitochondria Using Continuous Nycodenz Gradient Centrifugation; III. Analysis of Isolated Mitochondria; A. Assessing the Purity of Mitochondrial Preparations

B. Determining the Integrity of Mitochondrial PreparationsC. Assessment of Protein Localization to Mitochondria and to Subcompartments in the Organelle; D. Assessment of the Disposition of Proteins on Mitochondrial Membranes; IV. Isolation of Mitochondrial Outer Membranes, Contact Sites, and Inner Membranes; Acknowledgments; References; Chapter 3: Isolation and Subfractionation of Mitochondria from Plants; I. Introduction; II. Growth and Preparation of Plant Material; III. Isolation of Mitochondria; IV. Density Gradient Purification of Mitochondria; A. Sucrose; B. Percoll

C. Mini-Mitochondrial. Preparations for Screening Transgenic Lines

Notes:

Description based upon print version of record.

ISBN:

9786611052720

9781281052728

1281052728

9780080489377

0080489370

OCLC:

476057627