Cilia : motors and regulation / edited by Stephen M. King, Gregory J. Pazour.

Format:

Book

Contributor:

King, Stephen M.

Pazour, Gregory J.

Series:

Methods in cell biology ; v. 92.

Methods in cell biology, 0091-679X ; v. 92

Language:

English

Subjects (All):

Cilia and ciliary motion.

Cells--Motility.

Cells.

Physical Description:

1 online resource (245 p.)

Edition:

1st ed.

Place of Publication:

Burlington, Mass. : Academic Press, an imprint of Elsevier, 2009.

Language Note:

English

Summary:

Along with its companion volume on axonemal dynein-mediated motility, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. * Covers protocols for cilia and

Contents:

Cover; Methods in Cell Biology; Copyright Page; Contents; Contributors; Preface; Chapter 1 Bioinformatic Approaches to Dynein Heavy Chain Classification; I. Introduction; II. Methods; III. Materials; IV. Results and Discussion; Acknowledgments; References; Chapter 2 Identification and Characterization of Dynein Genes in; I. Introduction; II. Methods and Results; III. Discussion; Acknowledgments; References; Chapter 3 Purification of Axonemal Dyneins and Dynein-Associated Components from; I. Introduction; II. Detachment and Isolation of Flagella; III. Flagellar Demembranation

IV. Dynein Extraction from Flagellar AxonemesV. Fractionation of Flagellar Extracts; VI. Immunoprecipitation from Cell Body Extracts; VII. Conclusions; Acknowledgments; References; Chapter 4 Purification of Dyneins from Sperm Flagella; I. Introduction; II. Short Protocol for Isolation of Sperm Outer Arm Dynein from the Ascidian; III. Collection of Sperm; IV. Isolation of Sperm Flagella; V. Purification of Outer Arm Dyneins; VI. Regulation of Outer Arm Dynein; VII. Purification of Inner Arm Dyneins; VIII. Conclusion; References

Chapter 5 Protein Engineering Approaches to Study the Dynein Mechanism using a Dictyostelium Expression SystemI. Introduction; II. Preparation of Recombinant Dynein; III. FRET-Based Detection of Dynein's Conformational Changes; IV. Motility Assays; Acknowledgments; References; Chapter 6 Biophysical Measurements on Axonemal Dyneins; I. Introduction; II. Materials and Methods; III. Results and Discussion; IV. Summary; Acknowledgments; References; Chapter 7 Protein Electroporation into Chlamydomonas for Mutant Rescue; I. Introduction; II. Materials and Methods; III. Discussion; References

Chapter 8 Analysis of the Role of Nucleotides in Axonemal Dynein FunctionI. Introduction; II. Materials and Methods; III. Methods; IV. Results and Discussion; V. Outlook; Acknowledgments; References; Chapter 9 The Regulation of Dynein-Driven Microtubule Sliding in Chlamydomonas Flagella by Axonemal Kinases and Phosphatases; I. Introduction; II. The Chlamydomonas Experimental System: Axoneme Isolationand the Flagellar Mutants; III. In Vitro Microtubule Sliding Assays: The Method andDiscovery of Kinases and Phosphatases that Regulate Dynein

IV. Biochemical Analysis of Kinases and Phosphatases Localizedto the Chlamydomonas AxonemeV. Discussion of the Regulatory Model and Concluding Remarks; References; Chapter 10 Analysis of Redox-Sensitive Dynein Components; I. Introduction; II. Materials and Reagents; III. Methods; Acknowledgments; References; Chapter 11 Calcium Regulation of Ciliary Motility: Analysis of Axonemal Calcium-Binding Proteins; I. Introduction; II. How Does Calcium Enter Motile Cilia?; III. What Ciliary Proteins Serve as Calcium Sensors?; IV. How Does a Change in Calcium Concentration Modulate Motility?; V. Summary

References

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

9786612619649

9781282619647

1282619640

9780080961071

008096107X

OCLC:

659776480