Development of sea urchins, ascidians, and other invertebrate deuterostomes : experimental approaches / edited by Charles A. Ettensohn, Gregory A. Wray and Gary M. Wessel.

Format:

Book

Contributor:

Ettensohn, Charles A.

Wray, Gregory A.

Wessel, Gary M.

Series:

Methods in cell biology ; 74.

Methods in cell biology ; 74

Language:

English

Subjects (All):

Echinodermata.

Sea urchins.

Sea squirts.

Physical Description:

1 online resource (909 p.)

Place of Publication:

Amsterdam ; Boston : Elsevier Academic Press, c2004.

Language Note:

English

Summary:

This book provides a practical guide to experimental methods for studying the development invertebrate deuterostomes as animal model systems. The chapters provide detailed experimental protocols that cover a broad range of topics in modern experimental methods. Topics covered range from rearing embryos to the care of adult animals, while also presenting the basic experimental methods including light and electron microscopy, used to study gene expression, transgenics, reverse genetics, and genomic approaches.* Covers a wide range of methods, from classical embryology through modern geno

Contents:

Front Cover; Methods in Cell Biology; Copyright Page; Contents; Contributors; Preface; Dedication; Chapter 1. The Invertebrate Deuterostomes: An Introduction to Their Phylogeny, Reproduction, Development, and Genomics; I. Introduction; II. Phylogeny; III. Reproduction; IV. Development; V. Genomics; References; Part I: Procurement, Maintenance & Culture of Oocytes, Embryos, Larvae and Adults; Chapter 2. Care and Maintenance of Adult Echinoderms; I. Overview; II. Introduction; III. Adult Echinoderm Models: Their Reproductive Cycles and Gametogenesis; IV. Obtaining Adult Echinoderms

V. Maintenance of Adult Echinoderms in Land-Based SystemsVI. Care and Handling of Adult Echinoderms; References; Chapter 3. Echinoderm Eggs and Embryos: Procurement and Culture; I. Introduction; II. Method for Sea Urchins (Class Echinoidea); III. Method for Sea Stars (Class Asteroidea); IV. Method for Brittle Stars (Class Ophiuroidea, Order Ophiurida); V. Method for Sea Cucumbers (Class Holothuroidea); VI. Method for Interspecific Crosses; VII. Summary; References; Chapter 4. Culture of Echinoderm Larvae through Metamorphosis; I. Introduction; II. Materials for Culturing Algae and Larvae

III. Establishing Algal CulturesIV. Culturing Echinoderm Larvae; V. Metamorphosis and Beyond; References; Chapter 5. Obtaining and Handling Echinoderm Oocytes; I. Introduction; II. Experimental Preparation; III. Methods of Oocyte Collection; IV. Sea Urchin Oocytes Cultured In Vitro; V. Labeling of Oocyte Components; VI. Introduction of Experimental Substances into the Oocytes; VII. Concluding Remarks/Outlook; References; Chapter 6. Procurement and Culture of Ascidian Embryos; I. Overview; II. Ascidian Development and Metamorphosis; III. Larval Tissue Specification; IV. Experimental Techniques

V. ProtocolsReferences; Chapter 7. Culture of Adult Ascidians and Ascidian Genetics; I. Overview; II. Introduction; III. Culturing Ascidians; IV. Induced Developmental Mutants and Natural Variants/Mutants; V. Linkage Analysis and Mapping Genes in Ascidians; References; Chapter 8. Hemichordate Embryos: Procurement, Culture, and Basic Methods; I. Introduction; II. Procurement, Spawning, and Culture of S. kowalevskii; III. Procurement, Spawning, and Culture of Ptychodera flava; IV. Removal of Vitelline Envelope in S. kowalevskii; V. Whole-Mount In Situ Hybridization

VI. Preparation of Blocking Reagent for P. flavaVII. Materials and Reagents; References; Chapter 9. Cephalochordate (Amphioxus) Embryos: Procurement, Culture, and Basic Methods; I. Overview; II. Introduction; III. Obtaining Gametes of Branchiostoma floridae; IV. Raising Embryos; V. Manipulating Embryos; VI. Amphioxus Resources Available; VII. Concluding Remarks; References; Part II: Embryological Approaches; Chapter 10. Quantitative Microinjection of Oocytes, Eggs, and Embryos; I. Introduction; II. Methods; III. Equipment and Supplies (Prices are as of December, 2002); References

Chapter 11. Blastomere Isolation and Transplantation

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

9786611015862

9781281015860

1281015865

9780080496597

0080496598

OCLC:

301953486