Peptide-lipid interactions / edited by Sidney A. Simon, Thomas J. McIntosh.

Format:

Book

Contributor:

Simon, Sidney A.

McIntosh, Thomas J.

Series:

Current topics in membranes ; v. 52.

Current topics in membranes ; v. 52

Language:

English

Subjects (All):

Peptides.

Lipids.

Membrane proteins.

Membrane lipids.

Physical Description:

1 online resource (606 p.)

Place of Publication:

San Diego : Academic Press, c2002.

Language Note:

English

Summary:

This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion.

Contents:

Cover; Copyright Page; Contents; Contributors; Preface; Previous Volumes in Series; Part I: Biophysical Techniques for Analyzing Peptide-Lipid Interactions; Chapter 1. Peptide-Membrane Interactions Determined Using Site-Directed Spin Labeling; I. Introduction; II. Site-Directed Spin Labeling; III. Membrane Interactions of Basic Peptides Derived from Cell-Signaling Motifs; IV. Studies Directed at Channel-Forming and Other Membrane-Active Peptides; V. Conclusions; References; Chapter 2. Isothermal Titration Calorimetry for Studying Interactions between Peptides and Lipid Membranes

I. Introduction to Isothermal Titration CalorimetryII. Measurement of the Reaction Enthalpy and the Binding Isotherm; III. Binding Models for Analyzing Binding Isotherms; IV. Complex Membrane-Binding/Membrane-Perturbation Equilibria; V. Thermodynamics of the Membrane-Induced Coil-Helix Transition; VI. Thermodynamics of Peptide Binding to Curved and Planar Membranes; VII. Concluding Remarks; References; Chapter 3. Infrared Reflection-Absorption Spectroscopy of Lipids, Peptides, and Proteins in; I. Introduction; II. Experimental Approaches; III. Information from IRRAS Measurements

IV. IRRAS Applications to Lipid/Protein Langmuir FilmsV. Current State and Future Prospects for IRRAS; References; Chapter 4. Measuring the Depth of Amino Acid Residues in Membrane-Inserted Peptides by Fluorescence Quenching; I. Introduction; II. Determining Depth from Fluorescence Quenching by Empirical Inspection of Quenching Level; III. Determining Depth by Parallax Analysis; IV. Determining Depth by Distribution Analysis; V. Comparing Parallax Analysis and Distribution Analysis; VI. Investigating Membrane Protein Topography by Varying Fluorophore Depth Instead of Quencher Depth

VII. A Cautionary Note Concerning Quenching AnalysisVIII. Problems That Can Affect the Accuracy of Quenching Measurements; IX. Studies of Membrane-Inserted Peptides by Fluorescence Quenching; X. Conclusions; References; Chapter 5. Surface-Sensitive X-Ray and Neutron Scattering Characterization of Planar Lipid Model Membranes and Lipid/Peptide Interactions; I. Introduction; II. Data Inversion and Modeling in Reflectivity Measurements; III. Characterization of Planar Lipid Model Systems; References; Chapter 6. Lipid-Peptide Interaction Investigated by NMR; I. Introduction

II. Peptide Structure in the Membrane-Bound StateIII. Structure and Dynamics of the Lipid Matrix; IV. Future Directions; References; Chapter 7. Peptide-Lipid Interactions in Supported Monolayers and Bilayers; I. Peptide Insertion into Lipid Monolayers; II. Supported Bilayers; III. ATR-FTIR Spectroscopy of Peptides in Supported Bilayers; IV. Analysis of Fusion Peptide-Lipid Interactions; References; Part II: Theoretical and Computational Analyses of Peptide-Lipid Interactions; Chapter 8. Free Energy Determinants of Peptide Association with Lipid Bilayers; I. Introduction; II. Theory

III. Mean-Field Studies of Peptide-Membrane Systems

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

0-08-092585-5

1-281-00510-X

9786611005108

0-08-049096-4

OCLC:

808614911