Biotechnology annual review. Volume 13 / chief editor M. Raafat El-Gewely.

Format:

Book

Series:

Biotechnology Annual Review

Biotechnology annual review ; 13

Language:

English

Subjects (All):

Biotechnology.

Chemical engineering.

Physical Description:

1 online resource (379 p.)

Place of Publication:

Amsterdam ; New York : Elsevier, 2007.

Language Note:

English

Summary:

Biotechnology is a diverse, complex and rapidly evolving field. Students and experienced researchers alike face the challenges of staying on top of developments in their field of specialty and maintaining a broader overview of the field as a whole. Volumes containing competent reviews on a diverse range of topics in the field fulfill the dual role of broadening and updating biotechnologists' knowledge. The current volume is an excellent example of such a book. The topics covered range from classical issues in biotechnology - such as, recent advances in all-protein chromophore technology- to to

Contents:

Cover; Contents; Foreword; Editorial board; List of contributors; Chapter 1. Toward a new era in sequencing; Sequencing technologies of the next generation; Comparison: New fast sequencing technique vs. established Sanger method; Applications of the Genome Sequencer 20 system; References; Chapter 2. Optimizing scaleup yield for protein production: Computationally Optimized DNA Assembly (CODA) and Translation Engineering'; Introduction; Codon pair utilization patterns are highly biased; Over-represented codon pairs are translated slowly

Over-represented codon pairs occur at protein structural boundariesMisplaced and over-represented codon pairs can inhibit protein expression; Hot Rod' genes produce proteins at high levels; Applications of CODA technologies in modern biological drug, diagnostics and vaccine development; References; Chapter 3. Engineering, expression, purification, and production of recombinant thermolysin; Abbreviations; Introduction; Engineering and expression of recombinant thermolysin; Purification of recombinant thermolysin; Future perspectives; Conclusion; Acknowledgments; References

Chapter 4. Preparation of recombinant vaccinesIntroduction; The different systems to produce recombinant vaccines; Vaccine adjuvants, formulation and delivery; The use of virus-like particles as foreign antigen carrier systems; Conclusion; References; Chapter 5. Advances in antibody manufacturing using mammalian cells; Introduction; Upstream considerations; Downstream considerations; Conclusions; References; Chapter 6. Pharming and transgenic plants; Introduction; Plants have a high potential for pharmaceutical protein production

Which are the current limitations of the plant expression system?Optimization of plant production platforms for lower proteolytic activity and humanized glycosylation; Emerging plant expression systems for molecular farming; Conclusion; References; Chapter 7. Porous silicon protein microarray technology and ultra-/superhydrophobic states for improved bioanalytical readout; Preface; Introduction; Porous silicon - a novel 3D substrate for protein microarray technology; Effect of porous texture on microarray data quality; Superhydrophobic and ultrahydrophobic states on porous silicon

MS compatible protein arrays/dual-readout chipsAccelerated protein digestion and analyte enrichment in nanovials; Superhydrophobic MALDI-TOF MS target anchor chips; Conclusions; Acknowledgments; References; Chapter 8. Production of plasmid DNA for pharmaceutical use; Introduction; Plasmid production; Conclusions; References; Chapter 9. Potentials of phenolic molecules of natural origin and their derivatives as anti-HIV agents; Abbreviations used; Introduction; The AIDS and its therapy; Plant materials and extracts against HIV-1: Few examples

In silico approaches for the discovery of anti-HIV molecules from natural products

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-281-05988-9

9786611059880

0-08-055368-0

OCLC:

183327575