Evolutionary protein design / edited by Frances H. Arnold.

Format:

Book

Contributor:

Arnold, Frances Hamilton.

Series:

Advances in protein chemistry ; 55.

Advances in protein chemistry ; 55

Language:

English

Subjects (All):

Proteins.

Protein engineering.

Physical Description:

1 online resource (451 p.)

Place of Publication:

San Diego : Academic Press, c2001.

Language Note:

English

Summary:

This is the first high-quality, comprehensive overview of the field of evolutionary protein design. Topics include new protein design strategies, the structures of laboratory-evolved proteins, the evolution of non-natural enzyme functions, and the theory of laboratory evolution.

Contents:

Front Cover; Evolutionary Protein Design; Copyright Page; CONTENTS; PREFACE; Chapter 1. New Functions from Old Scaffolds: How Nature Reengineers Enzymes for New Functions; I. Introduction; II. Defining Protein Superfamilies; III. The Enolase Superfamily: A Paradigm for Understanding How Nature Evolved Enzymes for New Functions; IV. Studies of Other Superfamilies and Fold Classes; V. Conclusions; VI. References; Chapter 2. Evolution of Protein Functon by Domain Swapping; I. Introduction; II. Terminology; III. Evolution of Proteins in Nature by Domain Swapping

IV. Domain Swapping for Protein EngineeringV. Methodologies; VI. Perspective; References; Chapter 3. Rational Evolutionary Design: The Theory of In Vitro Protein Evolution; I. Introduction; II. Sequence Space and Fitness Landscapes; III. Modeling Directed Evolution; IV. Rational Evolutionary Design; V. Summary and Conclusions; References; Chapter 4. Temperature Adaptation of Enzymes: Lessons from Laboratory Evolution; I. Introduction; II. Influence of Temperature on Enzymes; III. Studies of Natural Extremophilic Enzymes; IV. Directed Evolution

V. Stability, Flexibility, and Catalytic ActivityVI. Why Are Proteins Marginally Stable?; VII. Why Are Thermophilic Enzymes Poorly Active at Low Temperature?; VIII. Conclusions; References; Chapter 5. Structural Analysis of Affinity Matured Antibodies and Laboratory-Evolved Enzymes; I. Introduction; II. Structural Studies of Antibody Affnity Maturation; III. Structural Studies of Enzyme Directed Evolution; IV. Conclusions; References; Chapter 6. Molecular Breeding: The Natural Approach to Protein Design; I. Introduction; II. The Need for Better Proteins

III. Strategies for Optimizing ProteinsIV. Screening Is Key; V. Beyond Proteins; VI. Concluding Remarks; References; Chapter 7. Analysis of Large Libraries of Protein Mutants Using Flow Cytometry; I. Introduction; II. Library Screening Technologies; III. Cell Surface Display Technologies; IV. Library Screening by Flow Cytometry; V. Concluding Remarks; References; Chapter 8. From Catalytic Asymmetric Synthesis to the Transcriptional Regulation of Genes: In Vivo and In Vitro Evolution of Proteins; I. Introduction and Scope of Review; II. Selecting and Evolving Therapeutic Human Antibodies

III. Directing Evolution at the Level of Chemical Mechanism: Aldolase Antibodies and Asymmetric CatalysisIV. Multiple Hapten Selection: Refining the Active Site of a Catalytic Antibody by In Vitro Selection; V. Selection and Evolution of Novel DNA-Binding Proteins: From Principles to Applications; References; Chapter 9. In Vitro Selection and Evolution of Proteins; I. Introduction; II. The Key to In Vitro Protein Evolution: Cell-Free Translation; III. In Vitro Selection Strategies; IV. Applications of Ribosome Display; V. Perspectives of Directed In Vitro Evolution; References; AUTHOR INDEX

SUBJECT INDEX

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

ISBN:

1-281-01064-2

9786611010645

0-08-049337-8

OCLC:

476067577