Advances in protein chemistry. Volume 74, Mechanisms and pathways of heterotrimeric G protein signaling / edited by Stephen R. Sprang.

Format:

Book

Contributor:

Sprang, Stephen R.

Series:

Advances in protein chemistry ; v. 74.

Advances in protein chemistry ; v. 74

Language:

English

Subjects (All):

G proteins.

Cellular signal transduction.

Physical Description:

1 online resource (262 p.)

Edition:

1st ed.

Place of Publication:

Amsterdam : Academic Press, 2007.

Summary:

This volume in the Advances in Protein Chemistry series features cutting-edge articles on topics in protein chemistry. This volume includes chapters on the structural basis of effector regulation and signal termination in heterotrimeric G N n nproteins; How do receptors activate G proteins; Some mechanistic insights into GPCR activation from detergent solubilized ternary complexes on beads; Activation of G protein coupled receptors; Kinetic analysis of g-protein-coupled receptor signaling using fluorescence resonance energy transfer in living cells; Regulation of Rho Guanine Nucleotide Exchange Factors (RhoGEFs) by G proteins."

Contents:

Cover; Contents; Preface; Chapter 1: Structural Basis of Effector Regulation and Signal Termination in Heterotrimeric Galpha Proteins; I. Introduction and Scope; II. A Selective Survey of Galpha Protein Structure and Function; III. Mechanisms of Effector Recognition and Regulation by GalphabullGTP; A. A Common Galpha:Effector Interface; B. Functional Consequences of Galpha:Effector Binding; IV. Signal Termination: The Mechanism of GTP Hydrolysis and Conformational Deactivation; A. Structure of the Ground State for GTP Hydrolysis; B. Reaction Trajectory for GTP Hydrolysis

V. Signal Termination Through GAPs and Effector GAP DomainsA. Deactivation of Galphaq by PLCbeta; B. RGS GAPs; C. Synergy Between RGS and Effector Domains; D. Deactivation of Galpha 13 by the alphaGAP Element of p115RhoGEF; VI. Conclusions; Acknowledgments; References; Chapter 2: How do Receptors Activate G Proteins?; I. Introduction; A. Structure of Heptahelical Receptors; B. Heterotrimeric G Protein Structure; II. Toward a Model of the Receptor-G Protein Complex; A. Structural Determinants of Receptor-G Protein Specificity; B. Point to Point Interactions Between Receptors and G Proteins

C. Current Approaches to Modeling the Receptor-G Protein ComplexIII. Molecular Basis for G Protein Activation; IV. Summary and Conclusions; References; Chapter 3: Some Mechanistic Insights into GPCR Activation from Detergent-Solubilized Ternary Complexes on Beads; I. Perspectives; II. Survey of Experimental Approaches and Representative Data; A. Flow Cytometric Approaches to Assess GPCR Function In Vivo; B. Rapid Mix Flow Cytometry; C. Modular Assembly of Molecular Complexes on Beads; III. Analysis of Soluble Receptor Ternary Complex Assemblies; A. General Considerations

B. Simple Ternary Complex Model: General ConsiderationsC. Application of Ternary Complex Model; D. Landscapes of G-Bead Assemblies Based on Application of Experimentally Derived Binding Constants to the Ternary Complex Model; E. Ternary Complex Analysis of Soluble Receptor Assemblies; F. Are Unique Conformational Changes in Receptors Elicited by Interactions with Ligands Resulting in Varied G Protein Interactions by the Ligand-Bound Receptor?; IV. Guanine Nucleotide Activation of Ternary Complex: Some Dynamic Aspects of Structure and Reactivity; A. General Considerations

B. Structural StudiesC. Some Dynamic Aspects of GPCR Activation; D. Modular Disassembly of the Ternary Complexes: Guanine Nucleotide Activation Causes the Rapid Separation of the GPCR and Ligand from the G Protein; E. GDP Activity?; F. Galpha Subunit Dissociation?; G. Outlook; Acknowledgments; References; Chapter 4: Activation of G Protein-Coupled Receptors; I. Introduction; II. Structural and Mechanistic Homology Among GPCRs; A. Rhodopsin as a Structural Model for GPCRs; B. GPCRs Activated by Diffusible Agonists; C. GPCR Oligomers; III. Conformational States

A. Basal Activity and Ligand Efficacy

Notes:

Description based upon print version of record.

ISBN:

1-281-07253-2

9786611072537

0-08-055269-2

OCLC:

476109179