Mitochondria / edited by Liza A. Pon, Eric A. Schon.

Format:

• Book

Contributor:

• Pon, Liza A.
• Schon, Eric A.

Series:

• Methods in cell biology ; 65.
• Methods in cell biology ; 65

Language:

English

Subjects (All):

• Cytology.
• Mitochondria.

Physical Description:

1 online resource (539 p.)

Place of Publication:

New York : Academic Press, 2001.

Language Note:

English

Summary:

This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs. The book is full of step-by-step ""how to"" methods with sample results, interpretations, and pitfalls. There is a unique set of appendices that include gene catalogs, mtDNA maps, and reagents for probing respiratory chain function. Finally, there are applications of state-of-the art microarray and gene chip technologies.Key Features* Isolation o

Contents:

• Cover; CONTENTS; Contributors; Preface; Chapter 1. Isolation and Subfractionation of Mitochondria from Animal Cells and Tissue Culture Lines; I. Introduction; II. General Properties of Mitochondrial Preparations; III. Mitochondria from Beef Heart; IV. Mitochondria from Rat Liver; V. Mitochondria from Muscle; VI. Synaptic and Nonsynaptic Mitochondria from Different Rat Brain Regions; VII. Mitochondria from Hamster Brown Adipose Tissue; VIII. Mitochondria from Insect Flight Muscle; IX. Mitochondria from Porcine Adrenal Cortex; X. Mitochondria from Human Platelets
• XI. Mitochondria from Fish LiverXII. Mitochondria from Sea Urchin Egg; XIII. Mitochondria and Kinetoplasts from Protozoa; XIV. Mitochondria and Mitoplasts from Cultured Cells; References; Chapter 2. Isolation and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae; I. Introduction; II. Isolation of Yeast Mitochondria of Different Purity; III. Analysis of Mitochondria Preparations; IV. Purification of Outer Membrane Vesicles from Yeast Mitochondria; References; Chapter 3. Isolation and Subfractionation of Mitochondria from Plants; I. Introduction
• II. Growth and Preparation of Plant MaterialIII. Isolation of Mitochondria by Differential Centrifugation; IV. Density Gradient Purification of Mitochondria; V. Mitochondrial Yield, Purity, Integrity, Storage, and Function; VI. Subfractionation of Mitochondrial Compartments; VII. Proteome Analysis; VIII. Analysis of in Organello Translation Products; IX. Conclusion; References; Chapter 4. Assessing Functional Integrity of Mitochondria in Vitro and in Vivo; I. Introduction; II. In Vitro Assays with Mitochondrial Preparations; III. Mitochondrial Functions within Living Cells; References
• Chapter 5. Assaying Mitochondrial Respiratory Complex Activity in Mitochondria Isolated from Human Cells and TissuesI. Introduction; II. Preparation of Mitochondrial Fractions; III. Spectrophotometric Measurement of the Activities of Individual Complexes I-IV; IV. Linked Assays; V. Applications of Techniques to Diagnostic Investigations; References; Chapter 6. In Vivo Measurements of Respiration Control by Cytochrome c Oxidase and in Situ Analysis of Oxidative Phosphorylation; I. Introduction; II. Measurements of Endogenous Respiration in Intact Cells
• III. KCN Titration of COX Activity in Intact CellsV. KCN Titration of Isolated COX Activity; IV. KCN Titration of Integrated COX Activity; VI. In Situ Analysis of Mitochondrial OXPHOS; References; Chapter 7. Assay of Mitochondrial ATP Synthesis in Animal Cells; I. Introduction; II. Methodological Considerations; III. Experimental Procedures; IV. ATP Synthesis in Cellular Models of Mitochondrial Diseases; References; Chapter 8. Measurement of Membrane Permeability and Permeability Transition of Mitochondria; I. Introduction; II. Procedures; III. Anticipated Results and Pitfalls; References
• Chapter 9. Assaying Actin-Binding Activity of Mitochondria in Yeast

Notes:

Description based upon print version of record.

ISBN:

• 9786611021078
• 9780080574455
• 0080574459
• 9781281021076
• 1281021075
• 9780125612852
• 0125612850
• 9780080522531
• 008052253X

OCLC:

808617316