Stem cell culture / edited by Jennie P. Mather.

Format:

Book

Contributor:

Mather, Jennie P., 1948-

Series:

Methods in cell biology ; v. 86.

Methods in cell biology, 0091-679X ; v. 86

Language:

English

Subjects (All):

Stem cells.

Stem cells--Research.

Cell culture.

Physical Description:

1 online resource (407 p.)

Edition:

1st ed.

Place of Publication:

Amsterdam : Academic Press, 2008.

Language Note:

English

Summary:

The purpose of this volume is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this

Contents:

Front Cover; Stem Cell Culture; Copyright Page; Contents; Contributors; Preface; Biography; Chapter 1: Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions; I. Introduction; II. Methods; III. Discussion; IV. Materials; References; Chapter 2: Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and Feeder-Free Conditions; I. Introduction; II. Methods and Materials; III. Results; IV. Discussion and Summary; References; Chapter 3: Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation

I. IntroductionII. Rationale; III. Methods; IV. Summary; References; Chapter 4: Culture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal Animal; I. Introduction; II. Rationale; III. Methods; IV. Materials; V. Discussion; References; Chapter 5: Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability; I. Introduction; II. Amniotic Fluid Cell Composition and Stem Cells; III. In Vitro Potential of Amniotic Fluid Stem Cells; IV. Ex Vivo and In Vivo Application of Amniotic Fluid Stem Cells; V. Discussion; References

Chapter 6: Method to Isolate Mesenchymal-Like Cells from Wharton's Jelly of Umbilical CordI. Introduction; II. Rationale; III. Methods; IV. Materials; V. UCMSCs and Culture Characteristics; VI. Discussion; References; Chapter 7: Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs); I. Introduction; II. Methods; III. Concluding Remarks; References; Chapter 8: Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance; I. Introduction; II. Epithelial-Mesenchymal Relationship; III. Stem Cells and Lineage Biology

IV. Liver ProcessingV. Fractionation of Liver Cell Subpopulations; VI. Feeder Cells; VII. Extracellular Matrix; VIII. Culture Media; IX. Monolayer Cultures of Cells; X. Three-Dimensional Systems; Appendix 1: Nomenclature, Glossary, and Abbreviations; Appendix 2: Details on the Preparation of Buffers/Reagents; Appendix 3: Commercial Sources of Reagents; Appendix 4: Sources of Primary and Secondary Antibodies for Immunoselection and for Characterization of the Cells; References; Chapter 9: Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo; I. Introduction

II. RationaleIII. Methods; IV. Materials; V. Discussion; VI. Summary; References; Chapter 10: Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells; I. Introduction; II. Rationale; III. Methods; IV. Materials; V. Discussion; VI. Summary; References; Chapter 11: Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue; I. Introduction; II. Isolation of hMSCs from Trabecular Bone; III. Isolation of hMSCs from Adipose Tissue; IV. Growth, Subculture, and Cryopreservation of Undifferentiated hMSCs; V. Surface Marker Characterization of hMSCs

VI. hMSC Differentiation Assays for Osteogenic, Adipogenic, and Chondrogenic Pathways

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

9786611309015

9781281309013

128130901X

9780080878041

0080878040

OCLC:

437209220