Methods in enzymology. Volume five hundred and seventy three, Enzymes of epigenetics, Part A / edited by Ronen Marmorstein.

Format:

Book

Contributor:

Marmorstein, Ronen, editor.

Series:

Methods in enzymology ; Volume 573.

Methods in Enzymology, 0076-6879 ; Volume 573

Language:

English

Subjects (All):

Epigenetics.

Enzymology.

Physical Description:

1 online resource (554 p.)

Edition:

First edition.

Place of Publication:

Amsterdam, [Netherlands] : Academic Press, 2016.

Summary:

Enzymes of Epigenetics, one of two new volumes in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.This volume covers research methods that are employed to study epigenetic regulation and includes structural, biochemical, molecular, biological, cellular.

Contents:

Front Cover; Enzymes of Epigenetics, Part A; Copyright; Contents; Contributors; Preface; Part I: Chromatin Structure and Histones; Chapter One: In Vitro Chromatin Assembly: Strategies and Quality Control; 1. Introduction; 2. Chromatin Reconstitution Strategies; 2.1. Fluorescent Labeling of the Histones and Refolding into Octamer; 2.1.1. Equipment; 2.1.2. Buffers and Reagents; 2.1.3. Procedure; 2.1.4. Notes; 2.2. End-Labeling of DNA; 2.2.1. Equipment; 2.2.2. Buffers and Reagents; 2.2.3. Procedure; 2.2.4. Notes; 2.3. Nucleosome Assembly; 2.3.1. Procedure; 2.3.2. Notes

2.4. Trinucleosome Assembly2.4.1. Equipment; 2.4.2. Buffers and Reagents; 2.4.3. Procedure; 2.4.4. Notes; 2.5. ``Microscale ́́or Step Dilution Method for Nucleosome Reconsitution; 3. Quality Control Strategies for In Vitro Reconstituted Chromatin; 3.1. Micrococcal Nuclease Digestion; 3.1.1. Equipment; 3.1.2. Buffers and Reagents; 3.1.3. Procedure; 3.1.4. Notes; 3.2. Analysis of MNase-Digested Products Via Bioanalyzer 2100; 3.2.1. Equipment; 3.2.2. Buffers and Reagents; 3.2.3. Procedure; 3.2.4. Notes; 3.3. Size-Exclusion Chromatography Coupled with Multiangle Light Scattering; 3.3.1. Equipment

3.3.2. Buffer and Reagents3.3.3. Procedure; 3.3.4. Notes; 3.4. Analytical Ultracentrifugation; 3.4.1. Equipment; 3.4.2. Buffers and Reagents; 3.4.3. Notes; 3.4.4. Procedure; 3.5. Atomic Force Microscopy Analysis of Trinucleosomes; 3.5.1. Equipment; 3.5.2. Buffers and Reagents; 3.5.3. Procedure; 3.5.4. AFM Data Processing; 3.5.5. Notes; 3.6. Small-Angle X-ray Scattering; 3.6.1. Equipment; 3.6.2. Buffers and Reagents; 3.6.3. Procedure; 3.6.4. Notes; 4. Summary and Conclusion; References

Chapter Two: Preparation, Crystallization, and Structure Determination of Chromatin Enzyme/Nucleosome Complexes1. Introduction; 2. Prepare Nucleosome Core Particles; 3. Prepare Chromatin Protein; 4. Purify Chromatin Complex; 4.1. Analytical Purification; 4.2. Preparative Purification; 5. Concentrate Chromatin Complex; 6. Crystallize Chromatin Complex; 6.1. Crystallization; 6.2. Fluorescent Labeling of Chromatin Protein for Cocrystallization with the Nucleosome; 7. Postcrystallization Soaks to Improve Diffraction; References

Chapter Three: Preparation of Recombinant Centromeric Nucleosomes and Formation of Complexes with Nonhistone Centromere P ...1. Introduction; 2. Preparation of Histones for CENP-A Nucleosome Assembly; 2.1. Expression of His-H2A and H2B Monomers and (CENP-A/H4)2 Heterotetramers; 2.1.1. Equipment; 2.1.2. Reagents; 2.1.3. Buffers; 2.1.4. Protocol; 2.2. Purification of (CENP-A/H4)2 Heterotetramer; 2.2.1. Equipment; 2.2.2. Reagents; 2.2.3. Buffers; 2.2.4. Protocol; 2.3. Purification of His-H2A, H2A, and H2B Monomers; 2.3.1. Equipment; 2.3.2. Reagents; 2.3.3. Buffers; 2.3.4. Protocol

2.4. Histone H2A/H2B Refolding

Notes:

Description based upon print version of record.

Includes bibliographical references at the end of each chapters and indexes.

Description based on online resource; title from PDF title page (ebrary, viewed July 22, 2016).

Description based on publisher supplied metadata and other sources.

ISBN:

9780128054321

0128054328

9780128053652

0128053658

OCLC:

953380241