Single molecule tools. Part B, Super-resolution, particle tracking, multiparameter, and force based methods / edited by Nils G. Walter.

Format:

Book

Contributor:

Walter, Nils G.

Series:

Methods in enzymology ; 475.

Methods in enzymology ; 475

Language:

English

Subjects (All):

Molecular biology--Methodology.

Molecular biology.

Enzymology.

Ultrastructure (Biology).

Physical Description:

1 online resource (745 p.)

Place of Publication:

London : Academic, 2010.

Language Note:

English

Summary:

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and ""just look at the thing"" as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rap

Contents:

Front Cover; Methods in Enzymology: Single Molecule Tools,Part B: Super-Resolution,Particle Tracking,Multiparameter, andForce Based Methods; Copyright Page; Contents; Contributors; Preface; Volume in Series; Chapter 1: Super-Accuracy and Super-Resolution: GettingAround the Diffraction Limit; 1. Overview: Accuracy and Resolution; 2. Getting Super-Accuracy; 3. Calculating Super-Accuracy; 4. Reaching Super-Resolution; 5. Future Directions; References; Chapter 2: Molecules and Methods for Super-Resolution Imaging; 1. Introduction; 2. Molecules for Super-Resolution Imaging

3. Selected Methods for Super-Resolution ImagingAcknowledgments; References; Chapter 3: Tracking Single Proteins in Live Cells Using Single-Chain Antibody Fragment-Fluorescent Quantum Dot Affinity Pair; 1. Introduction; 2. The Method: Targeting QDs via a Single-Chain Variable Fragment-Hapten Pair; 3. Functionalization of QDs; 4. Quantification of the Number of FL Molecules per FL-pc-QD; 5. Binding of FL-QDs to Anti-scFv Fusion Constructs; 6. DNA Constructs for Single FL-QD Imaging in Live Cells; 7. Single-Molecule Imaging of Live Mammalian Cells; Acknowledgments; References

Chapter 4: Recording Single Motor Proteins in the Cytoplasm of Mammalian Cells1. Introduction; 2. Basic Principles; 3. Labeling Molecular Motors for In Vivo Observations; 4. Instrumentation for Tracking Single Motors In Vivo; 5. Detailed Experimental Procedures; 6. Summary and Conclusions; Acknowledgments; References; Chapter 5: Single-Particle Tracking Photoactivated Localization Microscopy for Mapping Single-Molecule Dynamics; 1. Introduction; 2. Description of the sptPALM Method; 3. Labeling with Photoactivatable Fluorescent Probes; 4. Tracking Single Molecules

5. Experimental Example: sptPALM on a Membrane Protein6. Conclusions; References; Chapter 6: A Bird's Eye View: Tracking SlowNanometer-Scale Movements ofSingle Molecular Nano-assemblies; 1. Introduction; 2. DNA-Based Nanowalkers; 3. Considerations for Fluorescence Imaging of Slowly Moving Particles; 4. Single-Molecule Fluorescence Tracking of Nanowalkers; 5. Extracting Super-Resolution Position Information; 6. Concluding Remarks; Acknowledgments; References; Chapter 7: Anti-Brownian Traps for Studies on Single Molecules; 1. Theoretical Overview; 2. Anti-Brownian Trapping Systems

3. The ABEL Trap4. Applications; 5. Future Work: En Route to Single Fluorophores; Acknowledgments; References; Chapter 8: Plasmon Rulers as Dynamic Molecular Rulers in Enzymology; 1. Introduction; 2. The Basic Idea: Distance Dependence of Plasmon Coupling; 3. Hardware Needed for Single Particle Rayleigh Scattering Spectroscopy; 4. Which Readout-Intensity, Polarization, or Color?; 5. Ruler Calibration?; 6. Plasmon Ruler Assembly and Purification; 7. Example 1: Dynamics of DNA Bending and Cleavage by Single EcoRV Restriction Enzymes

8. Example 2: Spermidine Modulated Ribonuclease Activity Probed by RNA Plasmon Rulers

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

ISBN:

9786612770241

9781282770249

1282770241

9780123814838

0123814839

OCLC:

689052344