Mitochondrial function. Part A, Mitochondrial electron transport complexes and reactive oxygen species / edited by William S. Allison, Immo E. Scheffler.

Format:

Book

Contributor:

Allison, William S., 1935-

Scheffler, Immo E.

Series:

Methods in enzymology ; 456.

Methods in enzymology ; 456

Language:

English

Subjects (All):

Mitochondria.

Active oxygen.

Electron transport.

Protein kinases.

Phosphoprotein phosphatases.

Physical Description:

1 online resource (606 p.)

Place of Publication:

Amsterdam ; Boston : Academic Press, 2009.

Language Note:

English

Summary:

The first of two new volumes covering mitochondria, this volume presents modern methods that have been developed to examine mitochondrial electron transport chain complexes, iron-sulfur proteins and reactive oxygen species. These new techniques provide investigators with sensitive, original approaches to the study of disease states associated with mitochondrial malfunction. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequ

Contents:

Front Cover; Methods in Enzymology: Mitochondrial Function, Part A: Mitochondrial Electron Transport Complexes and Reactive Oxygen Species; Copyright Page; Contents; Contributors; Preface; Methods in Enzymology; Chapter 1: Visualizing Functional Flexibility by Three-Dimensional Electron Microscopy: Reconstructing Complex I of the Mitochondrial Respiratory Chain; 1. Introduction; 2. Random Conical Reconstruction; 3. Application of the Techniques to Complex I From Yarrowia Lipolytica; 4. Conclusion; Acknowledgments; References

Chapter 2: Correlated Light and Electron Microscopy/Electron Tomography of Mitochondria in Situ1. Introduction; 2. Light Microscopy; 3. Electron Microscope Tomography; Acknowledgments; References; Chapter 3: Studies of Complex I by Fourier Transform Infrared Spectroscopy; 1. Spectroscopic Studies of Complex I; 2. FTIR Difference Spectroscopy and Its Application to Complex I; 3. Preparation of Rehydrated Layers of Complex I for ATR-FTIR Spectroscopy; 4. Analysis of Rehydrated Complex I Layers by Perfusion-Induced ATR-FTIR Difference Spectroscopy

5. Analysis of Rehydrated Complex I Layers by Electrochemically-Induced ATR-FTIR Difference Spectroscopy6. Model Compounds and Strategies for Interpretation; 7. Example IR Difference Spectra of Complex I; Acknowledgments; References; Chapter 4: Electron Transfer in Respiratory Complexes Resolved by an Ultra-Fast Freeze-Quench Approach; 1. Introduction; 2. Ultra-Fast Freeze-Quench Setup; 3. Sample Preparation; 4. Calibration of the Freeze-Quench Setup; 5. Application of Freeze-Quench Approach for the Monitoring of Complex I Reduction by NADH; References

Chapter 5: Use of Ruthenium Photooxidation Techniques to Study Electron Transfer in the Cytochrome bc1 Complex1. Introduction; 2. Design and Synthesis of Ruthenium-Labeled Proteins; 3. Measurement of Interprotein Electron Transfer in a Ruthenium-Labeled Protein; 4. Measurement of Electron Transfer between Ruz-39-Cc and Cytochrome bc1; 5. Measurement of Electron Transfer within the Cytochrome bc1 Complex; Acknowledgments; References; Chapter 6: Mass Spectrometric Characterization of the Thirteen Subunits of Bovine Respiratory Complexes that are Encoded in Mitochondrial DNA; 1. Introduction

2. Analysis of Mitochondrial Membranes for mt-Proteins3. Analysis of Respiratory Complexes for mt-Proteins; 4. Mass Spectrometric Analysis of mt-Proteins; 5. Conclusions and Perspectives; References; Chapter 7: Tracing Human Mitochondrial Complex I Assembly by Use of GFP-Tagged Subunits; 1. Introduction; 2. Selection of Complex I Subunit for GFP Labeling; 3. Creation of Inducible Cell Line Expressing AcGFP1-tagged NDUFS3; 4. Tracing AcGFP1-Labeled NDUFS3 on BN-PAGE; Acknowledgments; References

Chapter 8: Two-Dimensional Native Electrophoresis for Fluorescent and Functional Assays of Mitochondrial Complexes

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

9786612285165

9781282285163

1282285165

9780080915333

0080915337

9780080877778

008087777X

OCLC:

299240537