Functional glycomics / edited by Minoru Fukuda.

Format:

Book

Contributor:

Fukuda, Minoru, 1945-

Series:

Methods in enzymology ; v. 417.

Methods in enzymology ; v. 417

Language:

English

Subjects (All):

Glycomics.

Glycolipids.

Physical Description:

1 online resource (482 p.)

Place of Publication:

Amsterdam ; Boston : Elsevier/Academic Press, c2006.

Language Note:

English

Summary:

In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the second of three in the Methods in Enzymology series, including Glycobiology (vol. 415) and Glycomics (vol. 416), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. These books are

Contents:

Front Cover; Functional Glycomics; Copyright Page; Table of Contents; Contributors; Preface; Volumes in Series; Section I: N-Glycan Function Revealed by Gene Inactivation; Chapter 1: Cytokine Sensitivity and N-GlycanProcessing Mutations; Introduction; Materials; Methods; Notes; Acknowledgments; References; Chapter 2: Phenotype Changes of Fut8 Knockout Mouse: Core Fucosylation Is Crucial for the Function of Growth Factor Receptor(s); Overview; Materials and Methods; References; Section II: Neural Cell Function

Chapter 3: Cellular and Molecular Analysis of Neural Development of Glycosyltransferase Gene Knockout MiceOverview; Acknowledgment; References; Chapter 4: Roles of Glycolipids in the Development and Maintenance of Nervous Tissues; Overview; Genetic Approaches; Fundamental Behavior Analysis; Surgical Approaches for Repair Potential; Morphological Approaches; References; Further Reading; Chapter 5: Analysis of Neural Cell Functions in Gene Knockout Mice: Electrophysiological Investigation of Synaptic Plasticity in Acute Hippocampal Slices; Overview; Acknowledgments; References

Chapter 6: Analysis of Neural Cell Function in Gene Knockout Mice: BehaviorIntroduction; Materials; Methods; Acknowledgments; References; Chapter 7: Electrophysiological Analysis of Interactions Between Carbohydrates and Transmitter Receptors Reconstituted in Lipid Bilayers; Overview; Acknowledgment; References; Section III: Notch Signaling and Muscular Dystrophy; Chapter 8: Methods for Analysis of O-Linked Modifications on Epidermal Growth Factor-Like and Thrombospondin Type 1 Repeats; Overview

Analysis of O-Fucose and O-Glucose Glycan Structure by Metabolic Radiolabeling Coupled with Alkali-Induced beta-EliminationGlycosylation Site Mapping by Mass Spectrometry; Acknowledgments; References; Chapter 9: Roles of O-Fucosyltransferase 1 and O-Linked Fucose in Notch Receptor Function; Overview; Enzyme Assay for O-Fucosyltransferase 1 Activity; Downregulation of Ofut1 by Use of RNAi Techniques; Quantification of the O-Fucose Level; Cell Surface Notch Staining; Notch Secretion Assay; Western Blotting for Notch; Notch-Ligand Binding Assay; Notch-OFUT1 Binding Assay; Acknowledgments

ReferencesChapter 10: Roles of O-Fucose Glycans in Notch Signaling Revealed by Mutant Mice; Overview; Glycosylation of Notch Receptors and Notch Ligands; Pofut1 Is an Essential Component of the Canonical Notch Signaling Pathway; Fringe Is a Modulator of Notch Signaling; beta4GalT-1 Is a Novel Regulator of Notch Signaling; Mutations in Other Glycosyltransferase Genes; Acknowledgments; References; Chapter 11: Defect in Glycosylation that Causes Muscular Dystrophy; Overview; Purification of alpha-DG; Method for Glycoprotein Enrichment and Western Blot Analysis (Laminin Overlay Assay)

Method for Laminin Overlay Assay

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

ISBN:

9786610751433

9781280751431

1280751436

9780080469003

0080469000

OCLC:

808601207