Glycomics / edited by Minoru Fukuda.

Format:

Book

Contributor:

Fukuda, Minoru, 1945-

Series:

Methods in enzymology ; v. 416.

Methods in enzymology ; v. 416

Language:

English

Subjects (All):

Carbohydrates.

Proteins.

Physical Description:

1 online resource (461 p.)

Place of Publication:

Amsterdam ; Boston : Elsevier/Academic Press, c2006.

Language Note:

English

Summary:

In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the second of three in the Methods in Enzymology series, including Glycobiology (vol. 415) and Functional Glycomics (vol. 417), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. Thes

Contents:

Front Cover; Glycomics; Copyright Page; Table of Contents; Contributors to Volume 416; Preface; Volume in Series; Section I: Carbohydrate-Protein Interactions-Physical Measurements; Chapter 1. Characterization of Specific Donor Binding to a1,4-N-Acetylhexosaminyltransferase EXTL2 Using Isothermal Titration Calorimetry; Chapter 2. NMR Analysis of Carbohydrate-Protein Interactions; Chapter 3. X-ray Crystal Structure Determination of Mammalian Glycosyltransferases; Section II: Phage Display Library

Chapter 4. Screening of Peptide-Displaying Phage Libraries to Identify Short Peptides Mimicking CarbohydratesChapter 5. Phage Display-Derived Human Antibodies Against Specific Glycosaminoglycan Epitopes; Section III: Glycosyltransferase Expression Profiling; Chapter 6. Comprehensive Enzymatic Characterization of Glycosyltransferases with a ß3GT or ß4GT Motif; Chapter 7. Autofluorescent Proteins for Monitoring the Intracellular Distribution of Glycosyltransferases; Chapter 8. Expression Profiling of Glycosyltransferases and Related Enzymes Using In Situ Hybridization

Chapter 9. Expression Profiling of Glycosyltransferases Using RT-PCRChapter 10. Expression Profiling of Glycosyltransferases and Related Enzymes Using Gene Microarrays; Section IV: CHO Mutants in Glycan Biosynthesis; Chapter 11. Lectin-Resistant CHO Glycosylation Mutants; Chapter 12. CHO Glycosylation Mutants: GPI Anchor; Chapter 13. CHO Glycosylation Mutants: Proteoglycans; Section V: Function of Sulfation; Chapter 14. Determination of Substrate Specificity of Sulfotransferases and Glycosyltransferases (Proteoglycans)

Chapter 15. Measuring the Activities of the Sulfs: Two Novel Heparin/Heparan Sulfate EndosulfatasesChapter 16. Determination of Chemokine-Glycosaminoglycan Interaction Specificity; Chapter 17. Identification of Proteoglycan-Binding Proteins; Chapter 18. Functions of Glycans Revealed by Gene Inactivation of L-Selectin Ligand Sulfotransferases in Mice; Section VI: Functions Revealed by Forced Expression or Gene Knockout; Chapter 19. Expression of Specific Carbohydrates by Transfection with Carbohydrate Modifying Enzymes

Chapter 20. N-Glycan Structure Analysis Using Lectins and an a-Mannosidase Activity AssayChapter 21. Targeted Disruption of the Gene Encoding Core 1ß1-3-Galactosyltransferase (T-Synthase) Causes Embryonic Lethality and Defective Angiogenesis in Mice; Chapter 22. Roles of Mucin-Type O-Glycans Synthesized by Core2ß1,6-N-Acetylglucosaminyltransferase; Chapter 23. Analysis of Leukocyte Rolling In Vivo and In Vitro; Chapter 24. Cell Type-Specific Roles of Carbohydrates in Tumor Metastasis; Author Index; Subject Index; Color Plate Section

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

ISBN:

9786611028756

9781281028754

1281028754

9780080549453

0080549454

OCLC:

476085764