Angiogenesis [electronic resource] : in vivo systems. Part B / editor, David A. Cheresh.

Format:

Book

Contributor:

Cheresh, David A.

Series:

Methods in enzymology ; v. 445.

Methods in enzymology ; v. 445

Language:

English

Subjects (All):

Neovascularization.

Blood-vessels--Growth.

Blood-vessels.

Physical Description:

1 online resource (460 p.)

Place of Publication:

Amsterdam ; Boston, MA : Academic, 2008.

Language Note:

English

Summary:

Understanding how angiogenesis ""works"" and how to control it will have massive implications on the management, treatments, and ultimately the prevention of many common (and not so common) diseases. Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over angiogenesis. Diseases that are angiogenesis-dependent result when blood vessels either grow excessively or insufficiently.* Tried-and-tested techniques wr

Contents:

Front Cover; Methods in Enzymology; Contributors; Preface; Chapter 1: Inflammation, Angiogenesis, and Lymphangiogenesis; 1. Introduction; 2. Angiogenesis and Lymphangiogenesis in Inflammation; 4. Commonly Used Markers of Blood Vessels and of Lymphatic Vessels; 6. Quantitating (Lymph)angiogenesis by Fluorescence-Activated Cell Sorting; Chapter 2: Color-Coded Fluorescent Mouse Models of Cancer Cell Interactions with Blood Vessels and Lymphatics; 3. Color-Coded Tumor-Host Models: GFP Nude Mouse; 4. Color-Coded Tumor-Host Models: Nestin-Driven-GFP Nude Mouse

5. Imaging Cancer Cell Trafficking in Lymphatics: Experimental Metastasis Model7. Protocol: Production of Fluorescent-Protein-Expressing Cancer Cell Lines; References; Chapter 3: Bone Marrow-Derived Vascular Progenitors and Proangiogenic Monocytes in Tumors; 1. Introduction; Chapter 4: Screening Phage-Display Peptide Libraries for Vascular Targeted Peptides; 1. Introduction; 2. Methods; Acknowledgments; References; Chapter 5: Avian Embryos: A Model for the Study of Primary Vascular Assembly in Warm-Blooded Animals; 1. Introduction; 2. Culture Insert Preparation

4. Construction of Microscope Incubator6. Embryonic Culture Insert/Dish Preparation; 8. Post-Incubation Fixation and Processing; 9. Whole-Mount Immunolabeling; 10. Plastic Embedding and Sectioning; Chapter 6: Mouse Models to Investigate Anti-Cancer Effects of VEGF Inhibitors; 4. Conclusions; References; Chapter 7: Molecular Imaging of Tumor Vasculature; 1. Introduction; 3. Molecular Imaging; 8. Summary; Chapter 8: Proteomic Mapping of the Vascular Endothelium In Vivo for Vascular Targeting; 1. Introduction; 2. Proteomics: An Overview; 3. Proteomic Methodologies; 7. Summary; References

Chapter 9: Development of Coronary Vessels1. Introduction; 2. Microdissection and Explant Culture of the Proepicardium; 3. Isolation of Total RNA from Individual PEs for Gene Expression Studies; 6. Methods to Study Coronary Vessel Development In Vivo; Chapter 10: Methods for Evaluating Uteroplacental Angiogenesis and Their Application Using Animal Models; 1. Introduction; 3. Methods to Evaluate and Quantify Placental Angiogenesis; Acknowledgments; References; Chapter 11: Intravital Microscopic Investigation of Leukocyte Interactions with the Blood Vessel Wall; 1. Introduction

2. Transillumination Intravital Microscopy3. Measuring Leukocyte Rolling, Adhesion, and Transmigration; 5. Conclusion; Chapter 12: Placental Remodeling of the Uterine Vasculature; 2. Isolation and Culture of Human Cytotrophoblasts; 3. Isolation and Culture of First-Trimester, Human Placental Villous Explants; 4. Identification of Cytotrophoblast-Modified Blood Vessels in Tissue Sections; 6. In Vitro Models of CTB Endovascular Invasion Using Explanted Spiral Arterioles; Chapter 13: An In Vivo Experimental Model for Postnatal Vasculogenesis; 1. Introduction

2. Isolation of Blood-Derived Endothelial Progenitor Cells

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

ISBN:

9786611928582

9786612168666

9781281928580

1281928585

9781282168664

1282168665

9780080921662

0080921663

OCLC:

808734013