Nitric oxide. Part G, Oxidative and nitrosative stress in redox regulation of cell signaling / edited by Enrique Cadenas, Professor and Chairman, Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, Los Angeles, CA, Lester Packer, Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, Los Angeles, CA.

Format:

Book

Contributor:

Cadenas, Enrique, editor.

Packer, Lester, editor.

Series:

Methods in enzymology ; v. 441.

Methods in enzymology ; volume 441

Language:

English

Subjects (All):

Oxidative stress.

Physiology, Pathological.

Nitric oxide--Physiological effect.

Nitric oxide.

Physical Description:

1 online resource (485 p.)

Place of Publication:

San Diego, California : Elsevier, [2008]

Language Note:

English

Summary:

The Nobel Prize was awarded in Physiology or Medicine in 1998 to Louis J. Ignarro, Robert F. Furchgott and Ferid Murad for demonstrating the signaling properties of nitric oxide. Nitric oxide (NO) is one of the few gaseous signaling molecules and is a key biological messenger that plays a role in many biological processes. NO research has led to new treatments for treating heart as well as lung diseases, shock and impotence. (Sildenafil, popularly known by the trade name Viagra, enhances signaling through NO pathways.) Scientists are currently testing whether NO can be used to stop the growt

Contents:

Front Cover; Nitric Oxide, Part G Oxidative and Nitrosative Stress in Redox Regulation of Cell Signaling; Copyright Page; Contents; Contributors; Volumes in Series; Chapter 1: Protein 3-Nitrotyrosine in Complex Biological Samples: Quantification by High-Pressure Liquid Chromatography/Electrochemical...; 1. Introduction; 2. Quantification of 3-NT in Proteins Using HPLC Separation and Electrochemical (EC) Detection; Acknowledgment; References

Chapter 2: Selective Fluorogenic Derivatization of 3-Nitrotyrosine and 3,4-Dihydroxyphenylalanine in Peptides: A Method Designed for Quantitative Proteomic Analysis1. Introduction; 3. Model Studies with 4-Methylcatechol and 2-Aminocresol; 5. Conclusions; Chapter 3: Nitroalkenes: Synthesis, Characterization, and Effects on Macrophage Activation; 1. Introduction; References; Chapter 4: In-Gel Detection of S-Nitrosated Proteins Using Fluorescence Methods; 1. Introduction; 2. Role of Trace Metal Ions in Ascorbate-Mediated Reduction of S-Nitrosothiols

3. Detection of Protein S-Nitrosation Using Fluorescent Labeling Methods4. Current Protocol for CyDye-Switch Method; 5. Difference Gel Electrophoresis Analysis of a Model Protein Mixture; 7. Fluorescence Detection of S-Nitrosated Proteins in Plasma; 9. Modifications of the Original Biotin-Switch Method; 10. Current Procedure for the Biotin-Switch Assay; 11. Conclusion; Chapter 5: The Arachidonate-Dependent Survival Signaling Preventing Toxicity in Monocytes/Macrophages Exposed to Peroxynitrite; 1. Introduction; 2. Materials and Methods; Acknowledgments; References

Chapter 6: Practical Approaches to Investigate Redox Regulation of Heat Shock Protein Expression and Intracellular Glutathione Redox State2. Nitric Oxide and Cellular Stress Response: Role of Vitagenes; 3. Material and Methods; 4. Results; References; Chapter 7: Monitoring Oxidative Stress in Vascular Endothelial Cells in Response to Fluid Shear Stress: From Biochemical Analyses to Micro- and Nanotechnologies; 3. Measurement of Endothelial ROS Generation in Response to Shear Stress; 4. Protein Oxidation and Nitration in Response to Shear Stress; 6. Summary

Chapter 8: Determination of S-Nitrosothiols in Biological and Clinical Samples Using Electron Paramagnetic Resonance Spectrometry with Spin Trapping1. Introduction; 3. Relative Yield of Spin-Trapped NO. from S-Nitrosoalbumin in Comparison with S-Nitrosoglutathione; 4. S-Nitrosoalbumin Recovery; 5. Potential Signal Contribution from N-Nitrosamines and Other Possible Sources of NO.; References; Chapter 9: Novel Method for Measuring S-Nitrosothiols Using Hydrogen Sulfide; 1. Introduction; 3. Specificity for H2S-Dependent Detection of RSNO

4. Comparison of H2S vs Tri-iodide and Copper/Cysteine Based Methods for RSNO Detection

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on online resource; title from PDF title page (ebrary, viewed August 19, 2014).

ISBN:

9786611749354

9781281749352

1281749354

9780080884356

0080884350

OCLC:

808732604