RNA interference / edited by David R. Engelke, John J. Rossi.

Format:

Book

Contributor:

Engelke, David R. (David Ross)

Rossi, John J.

Series:

Methods in enzymology ; v. 392.

Methods in enzymology, 0076-6879 ; v. 392

Language:

English

Subjects (All):

RNA--Handbooks, manuals, etc.

RNA.

RNA editing--Handbooks, manuals, etc.

RNA editing.

Gene silencing--Handbooks, manuals, etc.

Gene silencing.

Small interfering RNA--Handbooks, manuals, etc.

Small interfering RNA.

Physical Description:

1 online resource (487 p.)

Place of Publication:

San Diego : Elsevier Academic Press, c2005.

Language Note:

English

Summary:

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.RNA Interference will cover RNAi in non-vertebrates (plants, C. elegans, drosophila, and S. pombe), and Mammalian systems (human and non-human cells). This volume discusses e

Contents:

front cover; copyright; Table of Contents; Contributors to Volume 392; Methods in Enzymology; [1] Transgene-Induced RNA Interference as a Tool for Plant Functional Genomics; [2] Constructs and Methods for Hairpin RNA-Mediated Gene Silencing in Plants; [3] RNA Interference in Caenorhabditis elegans; [4] High-Throughput RNA Interference Screens in Drosophila Tissue Culture Cells; [5] Mechanistic Insights Aid Computational Short Interfering RNA Design; [6] Novel Methods for Expressing RNA Interference in Human Cells

[7] Delivery of Small Interfering RNA to Mammalian Cells in Culture by Using Cationic Lipid/Polymer-Based Transfection Reagents[8] Subcellular Distribution of Small Interfering RNA: Directed Delivery Through RNA Polymerase III Expression Cassettes and Localization by In Situ Hybridization; [9] Viral Delivery of Recombinant Short Hairpin RNAs; [10] Targeting Cellular Genes with PCR Cassettes Expressing Short Interfering RNAs; [11] Use of Short Hairpin RNA Expression Vectors to Study Mammalian Neural Development

[12] Analysis of T-Cell Development by Using Short Interfering RNA to Knock Down Protein Expression[13] Lentiviral Vector Delivery of Recombinant Small Interfering RNA Expression Cassettes; [14] Paradigms for Conditional Expression of RNA Interference Molecules for Use Against Viral Targets; [15] High-Throughput RNA Interference Strategies for Target Discovery and Validation by Using Synthetic Short Interfering RNAs: Functional Genomics Investigations of Biological Pathways; [16] RNA Interference In Vivo: Toward Synthetic Small Inhibitory RNA-Based Therapeutics

[17] Labeling and Characterization of Small RNAs Associated with the RNA Interference Effector Complex RITS[18] RNA Interference Spreading in C. elegans; [19] Human Dicer: Purification, Properties, and Interaction with PAZ PIWI Domain Proteins; [20] Delivery of siRNA and siRNA Expression Constructs to Adult Mammals by Hydrodynamic Intravascular Injection; [21] Analysis of Short Interfering RNA Function in RNA Interference by Using Drosophila Embryo Extracts and Schneider Cells; [22] Use of RNA Polymerase II to Transcribe Artificial MicroRNAs

[23] Adeno-Associated Virus Vectors for Short Hairpin RNA Expression[24] Simple, Robust Strategies for Generating DNA-Directed RNA Interference Constructs; Author Index; Subject Index

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

ISBN:

9786610633395

9781280633393

1280633395

9780080455396

0080455395

OCLC:

475997879