G protein pathways. Part C, Effector mechanisms / edited by Ravi Iyengar, John D. Hildebrandt.

Format:

Book

Contributor:

Hildebrandt, John D.

Iyengar, Ravi.

Series:

Methods in enzymology ; v. 345.

Methods in enzymology ; v. 345

Language:

English

Subjects (All):

G proteins.

Membrane proteins.

Physical Description:

1 online resource (695 p.)

Other Title:

Effector mechanisms

Place of Publication:

San Diego ; London : Academic Press, c2002.

Language Note:

English

Summary:

This third volume in the trio covering G proteins, features integrated approaches to studying G proteins. Methods pertaining to signaling mechanisms are presented, including theoretical and modeling approaches, biochemistry and molecular biology, and cell biology and physiology. The techniques for studying the structure and function of G proteins are important not only to those with specific research interests in them, but also endocrinologists and pharmacologists conducting research on signaling mechanisms that are increasingly understood to interact with G proteins.

Contents:

Front Cover; G Protein Pathways; Copyright Page; Table of Contents; Contributors to Volume 345; Preface; Volume in Series; Section I: Modeling Intracellular Signaling Pathways; Chapter 1. Use of Kinetikit and GENESIS for Modeling Signaling Pathways; Section II: Phosphodiesterases; Chapter 2. Assays of G Protein/cGMP-Phosphodiesterase Interactions; Chapter 3. Assaying G Protein-Phosphodiesterase Interactions in Sensory Systems; Section III: Calcium and Potassium Channels; Chapter 4. Studies of Endogenous G-Protein-Mediated Pathways in Neurons by Whole-Cell Electrophysiology

Chapter 5. Biochemical Approaches to Study Interaction of Calcium Channels with RGS12 in Primary Neuronal CulturesChapter 6. Assaying Phosphatidylinositol Bisphosphate Regulation of Potassium Channels; Section IV: Adenylyl Cyclases; Chapter 7. Purification of Soluble Adenylyl Cyclase; Chapter 8. Calcium-Sensitive Adenylyl Cyclase/Aequorin Chimeras as Sensitive Probes for Discrete Modes of Elevation of Cytosolic Calcium; Chapter 9. Kinetic Analysis of the Action of P-Site Analogs

Chapter 10. Expression, Purification, and Assay of Cytosolic (Catalytic) Domains of Membrane-Bound Mammalian Adenylyl CyclasesChapter 11. Identification of Putative Direct Effectors for Gαo, Using Yeast Two-Hybrid Method; Chapter 12. Identification of Transmembrane Adenylyl Cyclase Isoforms; Chapter 13. Functional Analyses of Type V Adenylyl Cyclase; Chapter 14. Photoaffinity Labeling of Adenylyl Cyclase; Chapter 15. Crystallization of Complex between Soluble Domains of Adenylyl Cyclase and Activated Gsα; Chapter 16. Generation of Adenylyl Cyclase Knockout Mice

Chapter 17. Construction of Soluble Adenylyl Cyclase from Human Membrane-Bound Type 7 Adenylyl CyclaseChapter 18. Genetic Selection of Regulatory Mutants of Mammalian Adenylyl Cyclases; Section V: Phospholipases and Lipid-Derived Products; Chapter 19. Expression and Characterization of Rat Brain Phospholipase D; Chapter 20. G-Protein-Coupled Receptor Regulation of Phospholipase D; Chapter 21. Analysis and Quantitation of Ceramide; Chapter 22. Assays for Phospholipase D Reaction Products

Chapter 23. Determination of Strength and Specificity of Membrane-Bound G Protein-Phospholipase C Association Using Fluorescence SpectroscopyChapter 24. Assays and Characterization of Mammalian Phosphatidylinositol 4,5-Bisphosphate-Sensitive Phospholipase D; Chapter 25. Characterization and Purification of Phosphatidylinositol Trisphosphate 5-Phosphatase from Rat Brain Tissues; Section VI: Small GTP-Binding Proteins; Chapter 26. Assay of Cdc42, Rac, and Rho GTPase Activation by Affinity Methods; Chapter 27. Assays of ADP-Ribosylation Factor Function

Chapter 28. Functional Characterization of p115 RhoGEF

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

ISBN:

9786611011222

9781281011220

1281011223

9780080496931

0080496938

OCLC:

808616294