Ribonucleases. Part A, Functional roles and mechanisms of action / edited by Allen W. Nicholson.

Format:

Book

Contributor:

Nicholson, Allen W.

Series:

Methods in enzymology ; v. 341.

Methods in enzymology ; v. 341

Language:

English

Subjects (All):

Ribonucleases.

Nucleases.

Physical Description:

1 online resource (771 p.)

Place of Publication:

San Diego, Calif. : Academic Press, c2001.

Language Note:

English

Summary:

This first of two volumes provides up-to-date, methods-related information on ribonuclease functions, assays, and applications. Chapter topics include the identification of, characterization of, and assays for secreted ribonucleases; viral ribonucleases, artificial and engineered ribonucleases, and ribozymes.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and re

Contents:

Front Cover; Ribonucleases; Copyright Page; Table of Contents; Contributors to Volume 341; Preface; Volume in Series; Section I: Ribonuclease Classification and Review; Chapter 1. A Natural Classification of Ribonucleases; Chapter 2. The Ribonuclease T1 Family; Chapter 3. Ribonuclease T2; Chapter 4. The Ribonuclease P Family; Section II: Ribonuclease Assays; Chapter 5. Fast, Facile, Hypersensitive Assays for Ribonucleolytic Activity; Chapter 6. Activity Staining for Detection of Ribonucleases Using Dried Agarose Film Overlay Method after Isoelectric Focusing

Chapter 7. Gel Renaturation Assay for RibonucleasesChapter 8. Analysis of Ribonucleases Following Gel Electrophoresis; Chapter 9. Ribonuclease Assays Utilizing Toluidine Blue Indicator Plates, Methylene Blue, or Fluorescence Correlation Spectroscopy; Chapter 10. Ribonuclease Activities of Trypanosome RNA Editing Complex Directed to Cleave Specifically at a Chosen Site; Chapter 11. Ribonuclease YI*, RNA Structure Studies, and Variable Single-Strand Specificities of RNases; Section III: Secreted Ribonucleases

Chapter 12. Bovine Pancreatic Ribonuclease A: Oxidative and Conformational Folding StudiesChapter 13. Purification of Engineered Human Pancreatic Ribonuclease; Chapter 14. Degradation of Double-Stranded RNA by Mammalian Pancreatic-Type Ribonucleases; Chapter 15. Seminal Ribonuclease: Preparation of Natural and Recombinant Enzyme, Quaternary Isoforms, Isoenzymes, Monomeric Forms; Assay for Selective Cytotoxicity of the Enzyme; Chapter 16. Angiogenin; Chapter 17. Eosinophil-Derived Neurotoxin; Chapter 18. Eosinophil Cationic Protein; Chapter 19. Deciphering the Mechanism of RNase T1

Chapter 20. Mitogillin and Related Fungal RibotoxinsChapter 21. RNase U2 and a-Sarcin: A Study of Relationships; Chapter 22. Secretory Acid Ribonucleases from Tomato, Lycopersicon esculentum Mill.; Chapter 23. Leczyme; Section IV: Ribonucleases H; Chapter 24. Prokaryotic Type 2 RNases H; Chapter 25. RNase H1 of Saccharomyces cerevisiae: Methods and Nomenclature; Chapter 26. Ribonucleases H of the Budding Yeast, Saccharomyces cerevisiae; Chapter 27. Human RNases H; Chapter 28. Assays for Retroviral RNase H; Section V: Synthetic Ribonucleases

Chapter 29. Sequence-Selective Artificial RibonucleasesChapter 30. RNA Cleavage by 1,4-Diazabicyclo[2.2.2]- octane-Imidazole Conjugates; Chapter 31. Preparation and Use of ZFY-6 Zinc Finger Ribonuclease; Section VI: Ribonucleolytic Nucleic Acids; Chapter 32. RNA Cleavage by the 10-23 DNA Enzyme; Chapter 33. Leadzyme; Chapter 34. Hammerhead Ribozyme Structure and Function in Plant RNA Replication; Chapter 35. Kinetic Analysis of Bimolecular Hepatitis delta Ribozyme; Chapter 36. Catalytic and Structural Assays for the Hairpin Ribozyme; Chapter 37. Intracellular Applications of Ribozymes

Section VII: Ribonuclease Inhibitors

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

ISBN:

9786611011208

9781281011206

1281011207

9780080496917

0080496911

OCLC:

808616159