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Applications of chimeric genes and hybrid proteins. Part C, Protein-protein interactions and genomics / edited by Jeremy Thorner, Scott D. Emr, John N. Abelson.
- Format:
- Book
- Series:
- Methods in enzymology ; v.328.
- Methods in enzymology ; v.328
- Language:
- English
- Subjects (All):
- Gene fusion.
- Proteins--Analysis.
- Proteins.
- Gene expression.
- Physical Description:
- 1 online resource (701 p.)
- Place of Publication:
- San Diego ; London : Academic Press, c2000.
- Language Note:
- English
- Summary:
- The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
- Contents:
- Front Cover; Applications of Chimeric Genes and Hybrid Proteins; Copyright Page; Table of Contents; Contributors to Volume 328; Preface; Volume in Series; Section I: Applications of Two-Hybrid Methods and Related Techniques for Analysis of Protein-Protein Interactions; Chapter 1. High-Throughput Screening for Protein-Protein Interactions Using Two-Hybrid Assay; Chapter 2. LexA-Based Two-Hybrid Systems; Chapter 3. Interaction Mating Methods in Two-Hybrid Systems; Chapter 4. Analysis and Identification of Protein-Protein Interactions Using Protein Recruitment Systems
- Chapter 5. A Bacterial Two-Hybrid System that Exploits a cAMP Signaling Cascade in Escherichia coliChapter 6. A Green Fluorescent Protein-Based Reverse Two- Hybrid System: Application to the Characterization of Large Numbers of Potential Protein- Protein Interactions; Chapter 7. Yeast Three-Hybrid System for Detecting Ligand Receptor Interactions; Chapter 8. Use of a Yeast Three-Hybrid System to Clone Bridging Proteins; Chapter 9. The Yeast Tribrid System: cDNA Expression Cloning of Protein Interactions Dependent on Posttranslational Modifications; Chapter 10. The Yeast Split-Hybrid System
- Section II: Other Approaches Using Chimeras for Identification and Analysis of Interactions of Proteins and Nucleic AcidsChapter 11. Mapping Protein-Protein Interaction Domains Using Ordered Fragment Ladder Far-Western Analysis of Hexahistidine-Tagged Fusion Proteins; Chapter 12. Mapping Specificity Determinants for Protein- Protein Association Using Protein Fusions and Random Peptide Libraries; Chapter 13. Selection of Genetic Agents from Random Peptide Aptamer Expression Libraries; Chapter 14. Detection of Protein-Protein Interactions by Protein Fragment Complementation Strategies
- Chapter 15. Monitoring Protein-Protein Interactions in Live Mammalian Cells by B-Galactosidase ComplementationChapter 16. Green Fluorescent Protein Chimeras to Probe Protein-Protein Interactions; Chapter 17. Protein Fusions to Coiled-Coil Domains; Chapter 18. Molecular Applications of Fusions to Leucine Zippers; Chapter 19. Using the Yeast Three-Hybrid System to Detect and Analyze RNA-Protein Interactions; Chapter 20. Principles and Applications of a Tat-Based Assay for Analyzing Specific RNA-Protein Interactions in Mammalian Cells; Section III: Phage Display and Its Applications
- Chapter 21. Phage Display for Selection of Novel Binding PeptidesChapter 22. Selectively Infective Phage Technology; Chapter 23. Use of Phage Display and Transition-State Analogs to Select Enzyme Variants with Altered Catalytic Properties: Glutathione Transferase as an Example; Chapter 24. Selecting and Evolving Functional Proteins in Vitro by Ribosome Display; Chapter 25. Yeast Surface Display for Directed Evolution of Protein Expression, Affinity, and Stability; Section IV: Construction of Hybrid Molecules by DNA Shuffiing and Other Methods
- Chapter 26. Methods for in Vitro DNA Recombination Random Chimeragenesis
- Notes:
- Description based upon print version of record.
- Includes bibliographical references and indexes.
- ISBN:
- 9786611011123
- 9781281011121
- 1281011126
- 9780080496832
- 0080496830
- OCLC:
- 476068501
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