RNA-ligand interactions [electronic resource] : Part B : molecular biology methods / edited by Daniel W. Celander, John N. Abelson.

Format:

Book

Contributor:

Celander, Daniel W. (Daniel Walter)

Abelson, John.

Series:

Methods in enzymology ; v. 318.

Methods in enzymology ; v. 318

Language:

English

Subjects (All):

Ligands (Biochemistry).

RNA.

Physical Description:

1 online resource (579 p.)

Place of Publication:

San Diego, CA ; London : Academic Press, c2000.

Language Note:

English

Summary:

RNA-Ligand Interactions, Part B focuses on molecular biology methods. Major topics covered include: solution probe methods, tethered-probe methodologies, in vitro affinity selection methodologies, genetic methodologies for detecting RNA-protein interactions, protein engineering methodologies useful for RNA-protein interaction studies, and cell biology methods.RNA-Ligand Interactions, Part A, its companion, VOLUME 317 focuses on structural biology methods.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is o

Contents:

Front Cover; Rna-Ligand Interactions; Copyright Page; Table of Contents; Contributors to Volume 318; Preface; Volumes in Series; Section I: Solution Probe Methods; Chapter 1. Probing RNA Structure and RNA-Ligand Complexes with Chemical Probes; Chapter 2. Methylene Blue-Mediated Cross-Linking of Proteins to Double-Stranded RNA; Chapter 3. Structure and Distance Determination in RNA with Copper Phenanthroline Probing; Chapter 4. Applications of Uranyl Cleavage Mapping of RNA Structure; Chapter 5. Identification of Messenger RNAs That Contain Inosine; Section II: Tethered-Probe Methodologies

A. Photochemical ReagentsChapter 6. Site-Specific 4-Thiouridine Incorporation into RNA Molecules; Chapter 7. Nucleoprotein Photo-Cross-Linking Using Halo- pyrimidine-Substituted RNAs; Chapter 8. Structure Determination by Directed Photo-Cross- Linking in Large RNA Molecules with Site-Specific Psoralen; Chapter 9. Photolabile Derivatives of Oligonucleotides as Probes of Ribosomal Structure; Chapter 10. Photoaffinity Cross-Linking and RNA Structure Analysis; B. Chemical Reagents; Chapter 11. Cleavage of RNA with Synthetic Ribonuclease Mimics

Chapter 12. Site-Specific Sulfhydryl Groups for Study of RNA Conformation via Disulfide Cross-LinkingChapter 13. Directed Hydroxyl Radical Probing Using Iron(II) Tethered to RNA; Section III: In Vitro Affinity Selection Methodologies; Chapter 14. In Vitro Selection of RNA Aptamers; Chapter 15. In Vitro Selection and Characterization of RNAs with High Affinity to Antibiotics; Chapter 16. Selection for RNA: Peptide Recognition through Sulfur Alkylation Chemistry; Chapter 17. In Vitro Selection of RNA Substrates for Ribonuclease P and Its Catalytic RNA

Chapter 18. RNA-Protein Interactions in Ribosomes. In Vitro Selection from Randomly Fragmented rRNAChapter 19. Optimized Synthesis of RNA-Protein Fusions for in Vitro Protein Selection; Section IV: Genetic Methodologies for Detecting RNA-Protein Interactions; A. Bacterial Systems; Chapter 20. Screening RNA-Binding Libraries by Transcriptional Antitermination in Bacteria; Chapter 21. Rapid Genetic Analysis of RNA-Protein Interactions by Translational Repression in Escherichia coli; Chapter 22. RNA Challenge Phages as Genetic Tools for Study of RNA-Ligand Interactions; B. Eukaryotic Systems

Chapter 23. Screening RNA-Binding Libraries Using Tat-Fusion System in Mammalian CellsChapter 24. Frameshifting Assay to Characterize RNA-Protein Interactions in Eukaryotic Cells; Chapter 25. Translational Repression Assay Procedure. A Method to Study RNA-Protein Interactions in Yeast; Chapter 26. Analysis of Low-Abundance Ribonucleoprotein Particles from Yeast by Affinity Chromatography and Mass Spectrometry Microsequencing; Chapter 27. Yeast Three-Hybrid System to Detect and Analyze RNA-Protein Interactions

Section V: Protein Engineering Methodologies Useful for RNA-Protein Interaction Studies

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

ISBN:

9786611011048

9781281011046

1281011045

9780080496757

008049675X

OCLC:

808616128