Methods in methane metabolism. Part B, Methanotrophy / edited by Amy C. Rosenzweig, Stephen W. Ragsdale.

Format:

Book

Contributor:

Rosenzweig, Amy C. (Amy Claire)

Ragsdale, Stephen W.

Series:

Methods in enzymology ; v. 495.

Methods in enzymology, 0076-6879 ; v. 495

Language:

English

Subjects (All):

Methanobacteriaceae.

Methane--Metabolism.

Methane.

Methylotrophic microorganisms.

Physical Description:

1 online resource (361 p.)

Edition:

1st ed.

Place of Publication:

Amsterdam [Netherlands] ; Boston [Mass.] : Elsevier/Academic Press, c2011.

Language Note:

English

Summary:

Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific commu

Contents:

Front Cover; Methods in Methane Metabolism, Part B: Methanotrophy; Copyright Page; Contents; Contributors; Preface; Volume in series; Chapter 1: Characterization of the Recombinant Pyrophosphate-Dependent 6-Phosphofructokinases from Methylomicrobium alcaliphilum 20Z andMethylococcus capsulatus Bath; 1. Introduction; 2. Methods; 3. Brief Overview of Current Knowledge of PPi-PFK in Microorganisms; 4. Properties of PPi-PFK from Methanotrophs; 5. Conclusion?; Acknowledgments; References; Chapter 2: Genes and Enzymes of Ectoine Biosynthesis in Halotolerant Methanotrophs; 1. Introduction

2. PCR-Based Approach for Identification of the Ectoine Biosynthesis Genes in Methanotrophs3. Transcriptional Regulation of the Ectoine Biosynthesis Genes; 4. Key Enzymes of Ectoine Biosynthesis; 5. Conclusion; Acknowledgments; References; Chapter 3: Facultative and Obligate Methanotrophs: How to Identifyand Differentiate Them; 1. Introduction; 2. Identification of Methanotrophic Capabilities in Novel Isolates; 3. Substrate Utilization Tests; 4. Tests for Culture Purity; References; Chapter 4: Approaches for the Characterization and Description of Novel Methanotrophic Bacteria

1. Introduction to Aerobic Methanotroph Characterization for Systematic Research and Other Purposes2. Broad Level Systematic Diversity; 3. Methodological Approaches; 4. Concluding Remarks; References; Chapter 5: Methylococcus capsulatus (Bath): From Genome to Protein Function, and Vice Versa; 1. Introduction; 2. Mapping of the Outer Membrane Proteome of M. capsulatus; 3. Inner Membrane Protein Complexes Studied with Blue-Native Polyacrylamide Gel Electrophoresis (BN-PAGE); 4. Visualization and Identification of M. capsulatus c-type Heme Proteins; Acknowledgments; References

Chapter 6: Functional Metagenomics of Methylotrophs1. Introduction; 2. Enrichment for Specific Functional Types Using SIP; 3. Creating a Metagenomic Database and Linking It to Functionality; 4. Ultrashort Read-Based Metatranscriptomics; 5. Conclusions and Future Perspectives; Acknowledgments; References; Chapter 7: Genetic Systems for Moderately Halo(alkali)philic Bacteria of the Genus Methylomicrobium; 1. Introduction; 2. Methods; 3. Outlook; Acknowledgments; References; Chapter 8: Development of a System for Genetic Manipulation of the Facultative Methanotroph Methylocella silvestris BL2

1. Introduction2. Growth of M. silvestris; 3. Introduction of Plasmid DNA by Conjugation and Electroporation; 4. Gene Deletion by Electroporation of Linear DNA; 5. Preparation of Competent Cells for Electroporation of M. silvestris; 6. Construction of Linear DNA for Gene Deletion by Homologous Recombination; 7. Electroporation; 8. Efficiency of Gene Deletion; 9. Case Study: Deletion of Isocitrate Lyase; 10. Complementation; 11. Phenotype of the Isocitrate Lyase Deletion Mutant; 12. Conclusions; References; Chapter 9: Mutagenesis of Soluble Methane Monooxygenase; 1. Introduction

2. Bacteriological Growth Media and Antibiotics

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

ISBN:

9786613164292

9781283164290

1283164299

9780123869067

0123869064

OCLC:

742333640