Methods in enzymology [electronic resource]. Volume five hundred and nine, Nanomedicine : infectious diseases, immunotherapy, diagnostics, antifibrotics, toxicology and gene medicine / volume editor, Nejat Duzgunes.

Format:

Book

Contributor:

Düzgüneş, Nejat.

Series:

Methods in enzymology ; v. 509.

Methods in enzymology, 0076-6879 ; v. 509

Language:

English

Subjects (All):

Nanomedicine.

Enzymes.

Physical Description:

1 online resource (475 p.)

Place of Publication:

San Diego : Academic Press ; Amsterdam : Elsevier, 2012.

Language Note:

English

Summary:

This volume in the Methods in Enzymology series comprehensively covers Infectious Diseases, Immunotheraphy, Gene Medicine, Diagnostics and Toxicology of Nanomedicine. With an international board of authors, this volume is split into sections that cover subjects such as Nanomedicines in Immunotherapy, Nanomedicine toxicity, and Diagnostic Nanomedicine. Comprehensively covers infectious diseases, immunotherapy, gene medicine, diagnostics, and toxicology of nanomedicineInternational board of authorsSplit into sections that cover subjects such as Nano

Contents:

Front Cover; Nanomedicine: Infectious Diseases, Immunotherapy, Diagnostics, Antifibrotics, Toxicology and Gene Medicine; Copyright; Contents; Contributors; Preface; Volumes in Series; Chapter 1: Enhanced Antiviral Activity of Acyclovir Loaded into Nanoparticles; 1 Introduction; 2 Preparation of Acyclovir-Loaded Nanoparticles and Fluorescent Nanoparticles; 2.1 Preparation and characterization of beta-CD-PACM inclusion complexes; 2.2 Preparation of nanoparticles; 2.3 Characterization of beta-CD-PACM nanoparticles; 2.3.1 Sizes and size distribution; 2.3.2 Morphology; 2.3.3 Surface charge

2.3.4 Drug loading and encapsulation efficiency2.3.5 Determination of the coumarin content in the fluorescent nanoparticles; 2.3.6 In vitro release kinetics; 2.4 Sterilization; 2.5 Physical stability of beta-CD-PACM nanoparticles; 3 Biocompatibility Assays; 3.1 Cell viability assay; 3.2 Evaluation of the hemolytic properties; 3.3 Complement activation assay; 3.4 Analysis of Intracellular ROS; 3.5 Evaluation of skin irritation potential of beta-CD-PACM nanoparticles on EpiVaginal Tissue Model; 4 Assessment of the Antiviral Activity; 4.1 HSV-1 infection and treatment of Vero cells

4.2 Virus titration by the plaque assay4.3 Inhibition of HSV-1 infection in Vero cells by the acyclovir complex with beta-CD-PACM nanoparticles; 4.4 Determination of acyclovir concentration in Vero cells; 4.5 Evaluation of cellular uptake of coumarin 6 beta-CD-PACM inclusion complexes; 5 Conclusions; Acknowledgments; References; Chapter 2: Gold manno-Glyconanoparticles for Intervening in HIV gp120 Carbohydrate-Mediated Processes; 1 Introduction; 2 Design, Preparation, and Characterization of manno-GNPs; 2.1 Preparation of manno-GNPs; 2.2 Characterization of manno-GNPs

3 Evaluation of manno-GNPs as Inhibitors of Carbohydrate-Mediated gp120 Interactions3.1 Binding study of manno-GNPs to 2G12 by SPR; 3.1.1 Direct binding of manno-GNPs to 2G12; 3.1.2 Competition experiments; 3.2 Study of the 2G12/GNP interaction by STD-NMR; 3.2.1 Titration of 2G12-oligomannoside mixtures with manno-GNPs; 3.2.2 2G12 deuteration; 3.2.3 Competition experiments; 3.2.4 Calculation of the dissociation constant; 4 Validation of manno-GNPs as Inhibitors of HIV-1/Cell Infection; 4.1 Effect of manno-GNPs on HIV-1 neutralization by 2G12 in cellular models

4.1.1 Determination of 2G12 neutralizing concentration4.1.2 Inhibition of manno-GNPs of 2G12-mediated HIV-1 neutralization; 4.2 Inhibition of DC-SIGN-mediated HIV-1 trans-infection of human T cells by manno-GNPs; 4.2.1 Cell culture and preparation of PBMCs from blood; 4.2.2 Trans-infection assay; Acknowledgments; References; Chapter 3: Nanoparticle-Mediated Targeted Delivery of Antiretrovirals to the Brain; 1 Introduction; 2 Preparation and Characterization of QD; 2.1 Quantum dots; 2.2 Synthesis of double-shelled CdSe/CdS/ZnS QDs

2.3 Aqueous dispersion of the QDs terminated with carboxyl groups

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

9786613612144

9781280582363

1280582367

9780123918703

0123918707

OCLC:

794328697