Methods in enzymology. Volume 570, Chemokines / edited by Tracy M. Handel.

Format:

Book

Contributor:

Handel, Tracy M., editor.

Series:

Methods in enzymology ; v. 570

Methods in Enzymology, 0076-6879 ; Volume 570

Language:

English

Subjects (All):

Chemokines.

Chemokines--Physiological effect.

Physical Description:

1 online resource (676 p.)

Edition:

First edition.

Place of Publication:

Amsterdam, [Netherlands] : Academic Press, 2016.

Summary:

Chemokines, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in chemokines, and includes sections on such topics as chemokine detection using receptors, tracking cellular responses to chemokines, recognition of GAG-bound chemokines, and the production of chemokine receptor complexes for structural and biophysical studies.- Continues the legacy of this premier serial with quality chapters authored by leaders in the field- Covers research methods in chemokines- Contains sections on such topics as chemokine detection using receptors, tracking cellular responses to chemokine, recognition of GAG-bound chemokines, and the production of chemokine receptor complexes for structural and biophysical studies

Contents:

Front Cover; Chemokines; Copyright; Contents; Contributors; Preface; Chapter One: Chemokine Detection Using Receptors Immobilized on an SPR Sensor Surface; 1. Surface Plasmon Resonance; 2. Chemokine Receptors: Members of the GPCR Family; 3. Chemokine Receptor Immobilization on the Sensor Chip; 4. Viral Particles as Chemokine Receptors Carriers in SPR; 4.1. Materials; 4.2. Method I: Generation of Retroviral Particles; 4.3. Method II: Generation of LVPs; 4.4. Method III: Titration of Viral Proteins; 4.5. Method IV: Determination of Chemokine Receptor Levels on the VP

4.6. Method V: Quantitation of Receptor Number on VPs4.7. Method VI: Attachment of VPs to Biosensor Surfaces; 5. SPR-Based Applications for Chemokine Receptors; 6. Conclusions; Acknowledgments; References; Chapter Two: Study of Chemotaxis and Cell-Cell Interactions in Cancer with Microfluidic Devices; 1. Introduction; 2. Methods; 2.1. Chemotaxis in Microfluidic Chemotaxis Chamber; 2.1.1. Making Microfluidic Chemotaxis Chamber; 2.1.2. Chemotaxis Assay for Differentiated HL-60 Cells; 2.2. Gradient Switching in Microfluidic Device; 2.2.1. Making a Gradient Switching Microfluidic Device

2.2.2. Chemotaxis Assay for Differentiated HL-60 Cells with Gradient Switch2.2.2.1. Cell Tracking and Data Analysis; 2.3. Cell-Cell Interaction in Tumor Microenvironment; 2.3.1. Creation of the Microfluidic Device; 3. Limitations; 4. Perspectives; 4.1. Shearing Force and Calculation of Chemotactic Force; 4.2. Cell Polarity Change Versus Cell Turning During a Gradient Change of Direction; 4.3. Potential Future Uses of Microfluidic Devices: Analysis of Circulating Tumor Cells; 4.4. Advantages of Using 3D Microbioreactors to Investigate Factors that Influence the Tumor Microenvironment

AcknowledgmentsReferences; Chapter Three: Generating Chemokine Analogs with Enhanced Pharmacological Properties Using Phage Display; 1. Introduction; 1.1. Chemokines in Health and Disease; 1.2. Chemokine Structure and Activity; 1.3. Applying Phage Display Technology to Chemokine Receptors; 2. Methods; 2.1. Library Design and Construction; 2.1.1. Choice of Phage Display System; 2.1.2. Introducing Library Diversity; 2.1.3. How Much Diversity Is Feasible?; 2.1.4. Partial Diversity; 2.1.5. Chemokine Walking: Exploring Diversity Step by Step; 2.1.6. Required Materials; 2.1.7. Library Construction

2.2. Selection of Libraries on Cells2.2.1. Considerations; 2.2.2. Cell Lines; 2.2.3. Required Materials; 2.2.4. Option 1: Selection for Internalizing Ligands; 2.2.5. Option 2: Selection for High-Affinity Cell Surface Binding; 3. Limitations; 3.1. The Atypical Chemokine Receptor DARC (ACKR1); 4. Perspectives; 4.1. A Chemokine-Based HIV Prevention Strategy; 4.2. New Tools to Study CCR5 Pharmacology and Cell Biology; 4.3. An Intrakine to Protect Cells from HIV Infection and a Vaccine Adjuvant; 4.4. A Prototypic Inhibitor of CX3CR1, the Fractalkine/CX3CL1 Receptor; References

Chapter Four: Methods for the Recognition of GAG-Bound Chemokines

Notes:

Description based upon print version of record.

Includes bibliographical references at the end of each chapters and index.

Description based on online resource; title from PDF title page (ebrary, viewed March 18, 2016).

ISBN:

9780128021958

0128021950

9780128021712

0128021713