Micro- and nanosystems for biotechnology / edited by J. Christopher Love.

Format:

Book

Contributor:

Love, J. Christopher, editor.

Series:

Advanced Biotechnology

Advanced biotechnology ; volume 2

Language:

English

Subjects (All):

Biotechnology.

Nanotechnology.

Microtechnology.

Physical Description:

1 online resource (305 p.)

Edition:

1st ed.

Place of Publication:

Weinheim, Germany : Wiley-VCH Verlag GmBH & Company KGaA, [2016]

Summary:

Emphasizing their emerging capabilities, this volume provides a strong foundation for an understanding of how micro- and nanotechnologies used in biomedical research have evolved from concepts to working platforms. Volume editor Christopher Love has assembled here a highly interdisciplinary group of authors with backgrounds ranging from chemical engineering right up to materials science to reflect how the intersection of ideas from biology with engineering disciplines has spurred on innovations. In fact, a number of the basic technologies described are reaching the market to advance the discovery and development of biopharmaceuticals. The first part of the book focuses on microsystems for single-cell analysis, examining tools and techniques used to isolate cells from a range of biological samples, while the second part is dedicated to tiny technologies for modulating biological systems at the scale of individual cells, tissues or whole organisms. New tools are described which have a great potential for (pre)clinical development of interventions in a range of illnesses, such as cancer and neurological diseases. Besides describing the promising applications, the authors also highlight the ongoing challenges and opportunities in the field.

Contents:

Cover; Title Page; Copyright; Contents; List of Contributors; About the Series Editors; Preface; Part I Microsystems for Single-Cell Analysis; Chapter 1 Types of Clinical Samples and Cellular Enrichment Strategies; 1.1 Introduction; 1.2 Types of Clinical Samples; 1.2.1 Solid Clinical Samples; 1.2.1.1 Cellular Subtypes Found in Solid Clinical Samples; 1.2.2 Liquid Clinical Samples and Cellular Subtypes; 1.2.2.1 Blood; 1.2.2.2 Bone Marrow; 1.2.2.3 Placental or Umbilical Cord Blood; 1.2.2.4 Urine; 1.2.2.5 Cerebrospinal Fluid (CSF); 1.2.2.6 Saliva

1.3 Sample Processing and Conventional Methods of Cell Enrichment1.3.1 Processing Solid Clinical Samples; 1.3.1.1 Processing Liquid Samples; 1.3.2 Cell Enrichment; 1.3.2.1 Laser Capture Microdissection (LCM); 1.3.2.2 Density Gradient Centrifugation; 1.3.2.3 Fluorescence-Activated Cell Sorting (FACS); 1.3.2.4 Magnetic Activated Cell Sorting (MACS); 1.3.2.5 CellSearchTM; 1.4 Microscale/Nanoscale Devices for Cellular Enrichment; 1.4.1 Filtration Approaches; 1.4.2 Hydrodynamic Mechanisms; 1.4.3 Surface Treatments; 1.4.4 Magnetophoresis; 1.4.5 Electrophoresis; 1.4.6 Acoustophoresis

1.4.7 Optical Tweezers/Traps1.5 Conclusion; References; Chapter 2 Genome-Wide Analysis of Single Cells and the Role of Microfluidics; 2.1 Motivation for Single-Cell Analysis of Genomes and Transcriptomes; 2.2 Single-Cell Genomics; 2.2.1 Major Technical Challenges; 2.2.2 Approaches to Single-Cell Genomics; 2.2.3 The Application and Impact of Microfluidics in Single-Cell Genomics; 2.3 Single-Cell Transcriptomics; 2.3.1 Major Technical Challenges; 2.3.2 Approaches to Single-Cell Transcriptomics; 2.3.3 Application and Impact of Microfluidics in Single-Cell Transcriptomics

2.4 The Future of Genome-Wide Single-Cell Analysis with Microfluidics2.4.1 Recent Advances in the Scalability of Single-Cell Analysis using Microfluidics; 2.4.2 How Microfluidics will Expand the Application-Space for Single-Cell Analysis; 2.4.3 Outstanding Hurdles for Genome-Wide Analysis of Single Cells; 2.4.4 Prospects for Clinical Applications of Microfluidic Single-Cell Analysis; Keywords and Definitions; References; Chapter 3 Cellular Immunophenotyping: Industrial Technologies and Emerging Tools; 3.1 Cellular Immune Status and Immunophenotyping; 3.2 Surface Marker Phenotyping

3.2.1 Multicolor Flow Cytometry3.2.2 Commercial Flow Cytometers; 3.2.3 High-Content Imaging Cytometry; 3.2.4 Current Limitations and Further Development of Flow Cytometry; 3.3 Functional Phenotyping; 3.3.1 ELISpot Technologies; 3.3.2 Multiplexed Immunoassays; 3.3.3 Emerging Single-Cell Technologies; 3.4 Conclusion; Keywords and Definitions; References; Chapter 4 Microsystem Assays for Studying the Interactions between Single Cells; 4.1 Introduction; 4.2 Advantages of Single-Cell Analysis over Conventional Assay Systems; 4.3 Analysis of Cell-Cell Communication between Pairs of Single Cells

4.3.1 Integrated Microfluidic Coculture Systems and Microwell Arrays

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on online resource; title from PDF title page (ebrary, viewed March 24, 2016).

ISBN:

9783527801329

3527801324

9783527801299

3527801294

OCLC:

944934602