The Handbook of Plant Metabolomics.

Format:

Book

Author/Creator:

Weckwerth, Wolfram.

Contributor:

Kahl, Guenter.

Series:

Molecular Plant Biology

Language:

English

Subjects (All):

Biochemistry--Technique--Handbooks, manuals, etc.

Gene expression--Technique--Handbooks, manuals, etc.

Metabolism.

Molecular biology--Technique--Handbooks, manuals, etc.

Molecular genetics.

Plant metabolites--Metabolism.

Plant metabolites.

Plants.

Local Subjects:

Biochemistry--Technique--Handbooks, manuals, etc.

Gene expression--Technique--Handbooks, manuals, etc.

Metabolism.

Molecular biology--Technique--Handbooks, manuals, etc.

Molecular genetics.

Physical Description:

1 online resource (452 p.)

Edition:

1st ed.

Place of Publication:

Hoboken : Wiley, 2013.

Language Note:

English

Summary:

Metabolomics is the analysis of all metabolites such as metabolic intermediates, hormones and so on, in a given system such as a cell, an organ or a whole organism. It is complementary to the genomics and proteomic analysis of a system and an essential building block towards the understanding of a whole biological system. Being a relatively new approach, a number of techniques have been recently developed in order to experimentally analyse metabolites and many researchers are expanding into this field. Knowledge about the metabolic status of a system is critical for its comprehensive unders

Contents:

The Handbook of Plant Metabolomics; Contents; Preface; List of Contributors; Part I Central Metabolism; 1 Metabolic Profiling of Plants by GC-MS; 1.1 Introduction; 1.2 Methods and Protocols; 1.2.1 Sample Preparation; 1.2.1.1 Sampling; 1.2.1.2 Homogenization and Extraction; 1.2.1.3 Procedure for Polar Extraction of Metabolites; 1.2.2 Chemical Derivatization: Methoxymation and Silylation; 1.2.2.1 Procedure for the Chemical Derivatization of Plant Extracts; 1.2.3 GC-MS Analysis; 1.2.3.1 Procedure to Acquire GC-MS Data; 1.2.4 Data Preprocessing and Export

1.2.4.1 Procedure for Postacquisition Data Preprocessing1.2.4.2 Data Analysis and Statistics; 1.2.4.3 Procedure for Postacquisition Data Analysis; 1.3 Applications of the Technology; 1.4 Perspectives; References; 2 Isotopologue Profiling - Toward a Better Understanding of Metabolic Pathways; 2.1 Introduction; 2.2 Methods and Protocols to Determine Isotopologues; 2.2.1 Mass Spectrometry; 2.2.2 Protocols for Isotopologue Profiling by GC-MS; 2.2.2.1 Protein-Bound Amino Acids; 2.2.2.2 Metabolic Intermediates and Polar Products; 2.2.2.3 Carbohydrates; 2.2.3 NMR Spectroscopy

2.2.4 Protocols for Isotopologue Profiling by NMR2.2.5 Deconvolution of Isotopologue Data; 2.2.6 Expanding the Metabolic Space by Retrobiosynthetic Analysis; 2.3 Applications; 2.3.1 Experiments Using [U-13C6] Glucose; 2.3.2 Experiments Using 13CO2; 2.4 Perspectives; References; 3 Nuclear Magnetic Resonance Spectroscopy for Plant Metabolite Profiling; 3.1 Introduction; 3.2 Methods and Protocols; 3.2.1 Sample Preparation; 3.2.1.1 Harvesting Plant Material; 3.2.1.2 Drying; 3.2.1.3 Extraction; 3.2.2 Data Acquisition; 3.2.3 Standard 1H-NMR Spectroscopy; 3.2.4 J-Resolved Spectroscopy

3.2.5 Data Analysis3.3 Applications; 3.3.1 1D 1H-NMR Spectroscopy; 3.3.2 2D NMR Spectroscopy; 3.3.2.1 J-Resolved Spectroscopy; 3.3.2.2 COSYand TOCSY; 3.3.2.3 HMBC and HMQC/HSQC; 3.3.2.4 NOESYor ROESY (CAMELSPIN); 3.3.2.5 DOSY; 3.3.3 Magic Angle Spinning; 3.4 Perspectives; References; 4 Comprehensive Two-Dimensional Gas Chromatography for Metabolomics; 4.1 Introduction; 4.2 Methods and Protocols; 4.2.1 Instrumentation; 4.2.2 Sample Preparation and Analysis; 4.2.3 Data Processing; 4.2.4 Metabolic Fingerprinting; 4.2.5 Quantitative Analysis of Selected Metabolites

4.3 Applications of the Technology4.3.1 Data Analysis; 4.3.2 Literature; 4.4 Perspectives; References; 5 MALDI Mass Spectrometric Imaging of Plants; 5.1 Introduction; 5.1.1 Sample Preparation; 5.1.2 Data Acquisition; 5.1.3 Data Processing; 5.2 Methods and Protocols; 5.2.1 Sample Preparation and Handling; 5.2.1.1 Intact Tissues; 5.2.1.2 Cryosectioning; 5.2.2 Matrix Deposition; 5.2.2.1 Paintbrush (Figure 5.2); 5.2.2.2 Sublimation (Figure 5.3); 5.2.3 MALDI-MS Imaging Measurement; 5.2.3.1 Bruker Ultraflex Instruments; 5.2.3.2 Waters MALDI Micro MX; 5.3 Imaging Intact Tissues and Objects

5.4 Future Perspectives

Notes:

Description based upon print version of record.

ISBN:

3-527-66988-4

1-299-27760-8

3-527-66989-2

OCLC:

842902628