Quantitative immunohistology : problems and solutions / Anthony S-Y Leong and Trishe Y-M Leong.

Format:

Book

Author/Creator:

Leong, Anthony S.-Y. (Anthony Siew-Yin), 1945-2011.

Contributor:

Leong, Trishe Y-M.

Series:

Immunology and immune system disorders.

Immunology and immune system disorders

Language:

English

Subjects (All):

Diagnostic immunohistochemistry.

Physical Description:

1 online resource (82 p.)

Edition:

1st ed.

Place of Publication:

Hauppauge, N.Y. : Nova Science Publishers, 2010.

Language Note:

English

Summary:

As immunohistochemistry enters its fourth decade as a diagnostic tool, this analytical procedure continues to experience poor reproducibility, albeit in specific situations. The problem becomes more important with the mounting pressure to employ the assay in a quantitative manner for the assessment of therapeutic and prognostic markers in a number of neoplasms. Qualitative applications of immunohistochemistry are well established and continue to increase with the range of sensitive antibodies and detection systems available, however, numerous variables that influence the immunoexpression of proteins in formalin-fixed tissue continue to exist in the pre-analytical and analytical phases of the test procedure. This book emphasizes these problems in detail and suggests possible solutions.

Contents:

The immunohistological assay : a total test approach

Pre-analytical variables : tissue preservation, transportation, fixation and tissue processing

Analytical variables : antigen retrieval, antibodies, detection systems and controls

Post-analytical phase

Receptor detection for targeted therapies

Interobserver variability in quantitative immunohistology.

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on print version record.

ISBN:

9781616686116

1616686111

OCLC:

923662102