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Analytical molecular biology : quality and validation / edited by Ginny C. Saunders and Helen C. Parkes ; with a foreword by S.B. Primrose.
- Format:
- Book
- Series:
- Valid Analytical Measurement
- Valid Analytical Measurement ; v.8
- Language:
- English
- Subjects (All):
- Molecular biology.
- Biochemistry.
- Physical Description:
- 1 online resource (209 p.)
- Edition:
- 1st ed.
- Place of Publication:
- Cambridge : Published for Laboratory of the Government Chemist by the Royal Society of Chemistry, 1999.
- Language Note:
- English
- Summary:
- In spite of the wide variety and complexity of biological materials, nucleic acids are ubiquitous. DNA is becoming the bioanalyte of choice due to the vast amount of information embedded in its sequence, its robust chemical nature and the range of highly sensitive analytical techniques that have been developed. The results of such analyses can have an important impact on our society both commercially and in terms of the quality of life. Absolute confidence in the data generated is therefore of the utmost importance. This book, produced by LGC as part of the VAM (Valid Analytical Measurement)
- Contents:
- An introduction to analytical molecular biology
- Quality in the analytical molecular biology laboratory
- DNA extraction
- Quantification of total DNA by spectroscopy
- PCR: factors affecting reliability and validity
- Inhibitors and enhancers of PCR
- Random amplified polymorphic DNA analysis
- Development of multiplex PCR
- Membrane hybridisation
- Automated fluorescent DNA cycle sequencing.
- Notes:
- Description based upon print version of record.
- Includes bibliographical references and index.
- ISBN:
- 9781847559296
- 1847559298
- OCLC:
- 843640699
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