Animal cell culture : essential methods / editor, John M. Davis.

Format:

Book

Contributor:

Davis, John, 1952-

Series:

Essential Methods

Language:

English

Subjects (All):

Cell culture--Technique.

Cell culture.

Tissue culture--Technique.

Tissue culture.

Physical Description:

1 online resource (380 p.)

Edition:

1st ed.

Place of Publication:

Hoboken, N.J. : John Wiley & Sons Inc., 2011.

Language Note:

English

Summary:

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible.This book is

Contents:

CONTENTS; Contributors; Preface; Abbreviations; Protocols; 1 The Cell Culture Laboratory; 1.1 Introduction; 1.2 Methods and approaches; 1.3 Troubleshooting; Acknowledgements; References; 2 Sterilization; 2.1 Introduction; 2.2 Methods and approaches; 2.3 Troubleshooting; References; 3 Microscopy of Living Cells; 3.1 Introduction; 3.2 Methods and approaches; 3.3 Troubleshooting; Acknowledgements; References; 4 Basic Techniques and Media, the Maintenance of Cell Lines,and Safety; 4.1 Introduction; 4.2 Methods and approaches; 4.3 Troubleshooting; Acknowledgements; References

5 Development and Optimization of Serum- and Protein-freeCulture Media5.1 Introduction; 5.2 Methods and approaches; 5.3 Troubleshooting; 5.4 Conclusion; Acknowledgements; References; 6 Cryopreservation and Banking of Cell Lines; 6.1 Introduction; 6.2 Methods and approaches; 6.3 Troubleshooting; Acknowledgements; References; 7 Primary Culture of Specific Cell Types and the Establishmentof Cell Lines; 7.1 Introduction; 7.2 Methods and approaches; 7.3 Troubleshooting; References; 8 Cloning; 8.1 Introduction; 8.2 Methods and approaches; 8.3 Troubleshooting; References

9 The Quality Control of Animal Cell Lines and the Prevention,Detection and Cure of Contamination9.1 Introduction; 9.2 Methods and approaches; 9.3 Troubleshooting; References; 10 Systems for Cell Culture Scale-up; 10.1 Introduction; 10.2 Methods and approaches; 10.3 Troubleshooting; References; 11 Good Laboratory Practice in the Cell Culture Laboratory; 11.1 Introduction; 11.2 Background to GLP; 11.3 General GLP principles; 11.4 Study performance; 11.5 Good Manufacturing Practice; 11.6 Summary; References; Index

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on metadata supplied by the publisher and other sources.

ISBN:

9786613239365

9781283239363

1283239361

9780470669822

0470669829

9780470669815

0470669810

OCLC:

711780326