Molecular biology and genomics / Cornel Mulhardt ; translated by E.W. Beese.

Format:

Book

Author/Creator:

Mülhardt, Cornel.

Series:

Experimenter series.

The experimenter series

Standardized Title:

Experimentator. English

Language:

English

Subjects (All):

Molecular biology--Research--Methodology.

Molecular biology.

Genomics--Research--Methodology.

Genomics.

Physical Description:

1 online resource (273 p.)

Edition:

1st ed.

Place of Publication:

Burlington, MA : Academic Press, c2007.

Language Note:

English

Summary:

Never before has it been so critical for lab workers to possess the proper tools and methodologies necessary to determine the structure, function, and expression of the corresponding proteins encoded in the genome. Mulhardt's Molecular Biology and Genomics helps aid in this daunting task by providing the reader with tips and tricks for more successful lab experiments. This strategic lab guide explores the current methodological variety of molecular biology and genomics in a simple manner, addressing the assets and drawbacks as well as critical points. It also provides short and precise

Contents:

Front Cover; Molecular Biology and Genomics; Copyright Page; Table of Contents; Foreword; Foreword to the First Edition; Acknowledgments; Chapter 1 What Is Molecular Biology?; 1.1 The Substrate of Molecular Biology: The Molecular World for Beginners; 1.2 What Is Required for This Work?; 1.3 Safety in the Laboratory; Chapter 2 Fundamental Methods; 2.1 Differences in Nucleic Acids; 2.2 Precipitation and Concentration of Nucleic Acids; 2.2.1 Alcohol Precipitation; 2.2.2 Concentrators; 2.2.3 Savant Speed Vac; 2.2.4 Salting Out; 2.3 Purification of Nucleic Acids; 2.3.1 Phenol-Chloroform Extraction

2.3.2 Precipitation Using Polyethylene Glycol2.3.3 Protein-Binding Filter Membranes; 2.3.4 Anion-Exchange Columns; 2.3.5 Glass Milk; 2.3.6 Cesium Chloride Density Gradient; 2.3.7 Dialysis; 2.4 Determining the Concentration of Nucleic Acid Solutions; 2.4.1 Optical Density Measurements with the Aid of Absorption Spectrometry; 2.4.2 Determination of Concentration by Means of Agarose Gels; 2.4.3 Dot Quantification; 2.4.4 Fluorometric Determination; 2.4.5 Nucleic Acid Dipsticks; 2.4.6 Enzymatic Evidence; 2.5 Methods of DNA Preparation; 2.5.1 Preparation of Plasmid DNA on a Small Scale

2.5.2 Preparation of Plasmid DNA on a Large Scale2.5.3 Bacterial Media; 2.5.4 Preparation of Phage DNA; 2.5.5 Preparation of Single-Stranded DNA with the Aid of Helper Phages; 2.5.6 Preparation of Genomic DNA; Chapter 3 The Tools; 3.1 Restriction Enzymes; 3.1.1 Nomenclature; 3.1.2 The Activity Test; 3.1.3 Making a Restriction Digestion; 3.1.4 Difficulties Associated with Restriction Digestion; 3.1.5 Works of Reference for Restriction Digestion; 3.2 Gels; 3.2.1 Agarose Gels; 3.2.2 Isolating DNA Fragments from Agarose Gels; 3.2.3 Polyacrylamide Gels

3.2.4 Isolating DNA Fragments from Polyacrylamide Gels3.2.5 Pulse-Field Gel Electrophoresis; 3.2.6 Capillary Electrophoresis; 3.3 Blotting; 3.3.1 Southern Blots; 3.3.2 Northern Blots; 3.3.3 Dot Blots and Slot Blots; Chapter 4 The Polymerase Chain Reaction; 4.1 Standard Polymerase Chain Reaction; 4.2 Suggestions for Improving the Polymerase Chain Reaction; 4.2.1 Nested Polymerase Chain Reaction; 4.2.2 Multiplex Polymerase Chain Reaction; 4.2.3 Amplification of Longer DNA Fragments; 4.3 PCR Applications; 4.3.1 Reverse Transcription-Polymerase Chain Reaction

4.3.2 Rapid Amplification of cDNA Ends4.3.3 Amplification of Coincidental Products; 4.3.4 Classic Quantitative Polymerase Chain Reaction; 4.3.5 Real-Time Quantitative Polymerase Chain Reaction; 4.3.6 Inverse Polymerase Chain Reaction; 4.3.7 Biotin-RAGE Method and Supported Polymerase Chain Reaction; 4.3.8 Mutagenesis with Modified Primers; 4.3.9 Amplification Refractory Mutation System; 4.3.10 In Situ Polymerase Chain Reaction; 4.3.11 Cycle Sequencing; 4.3.12 cDNA Synthesis; 4.3.13 Single-Cell Polymerase Chain Reaction; Chapter 5 RNA; 5.1 Inactivating RNases; 5.2 Methods of RNA Isolation

5.2.1 Single-Step Method

Notes:

Translation of: Experimentator : Molekularbiologie. 4th ed.

Includes bibliographical references and index.

ISBN:

1-280-74685-8

9786610746859

0-08-046766-0

OCLC:

476031730