Insect histology : practical laboratory techniques / Pedro Barbosa, Deborah L. Berry, Christina S. Kary.

Format:

Book

Author/Creator:

Barbosa, Pedro, 1944- author.

Berry, Deborah L., author.

Kary, Christina S., author.

Language:

English

Subjects (All):

Entomology--Laboratory manuals.

Entomology.

Histology--Laboratory manuals.

Histology.

Physical Description:

1 online resource (397 p.)

Edition:

Second edition.

Place of Publication:

West Sussex, England : John Wiley & Sons, 2015.

Language Note:

English

Summary:

This title is a much needed update of Barbosa''s self-published Manual of Basic Techniques in Insect Histology. It is a laboratory manual of ''traditional'' and ''modern'' insect histology techniques, completely revised using cutting-edge methodology carried out today and includes new immunohistochemical techniques not previously looked at. Insect Histology is designed as a resource for student and professional researchers, in academia and industry, who require basic information on the procedures that are essential for the histological display of the tissues of insects and related organisms.

Contents:

Problems of sclerotized chitin : softening insect cuticle

Fixation

Dehydration, clearing, and embedding

Staining

Immunohistochemical staining

Use of genetic markers in insect histology

Fluorescence

Mounting

Preparation of whole mounts

Whole mount staining

Preparation of insect tissues

Preparation of genitalia, mouthparts, and other body parts

Preparation of chromosomes

Appendix : dissecting fluids and insect saline solutions.

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on print version record.

ISBN:

9781444336955

1444336959

9781118876114

1118876113

9781118875001

1118875001

9781118875711

1118875710

OCLC:

892799535