Analyzing biomolecular interactions by mass spectrometry / edited by Jeroen Kool and Wilfried M. A. Niessen.

Format:

Book

Contributor:

Kool, Jeroen, editor.

Niessen, Wilfried M. A., editor.

Language:

English

Subjects (All):

Biomolecules--Analysis.

Analytical chemistry--Quantitative.

Physical Description:

1 online resource (403 p.)

Edition:

1st ed.

Place of Publication:

Weinheim, Germany : Wiley-VCH Verlag GmbH & Co., 2015.

Language Note:

English

Summary:

This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomi

Contents:

""Analyzing Biomolecular Interactions by Mass Spectrometry""; ""Contents ""; ""List of Contributors ""; ""Preface ""; ""Abbreviations ""; ""Chapter 1 Introduction to Mass Spectrometry, a Tutorial ""; ""1.1 Introduction ""; ""1.2 Figures of Merit ""; ""1.2.1 Introduction ""; ""1.2.2 Resolution ""; ""1.2.3 Mass Accuracy ""

""1.2.4 General Data Acquisition in MS """"1.3 Analyte Ionization ""; ""1.3.1 Introduction ""; ""1.3.2 Electrospray Ionization ""; ""1.3.3 Matrix-Assisted Laser Desorption Ionization ""; ""1.3.4 Other Ionization Methods ""; ""1.3.5 Solvent and Sample Compatibility Issues ""

""1.4 Mass Analyzer Building Blocks """"1.4.1 Introduction ""; ""1.4.2 Quadrupole Mass Analyzer ""; ""1.4.3 Ion-Trap Mass Analyzer ""; ""1.4.4 Time-of-Flight Mass Analyzer ""; ""1.4.5 Fourier Transform Ion Cyclotron Resonance Mass Spectrometer ""; ""1.4.6 Orbitrap Mass Analyzer ""

""1.4.7 Ion Detection """"1.5 Tandem Mass Spectrometry ""; ""1.5.1 Introduction: ""Tandem-in-Time"" and ""Tandem-in-Space"" ""; ""1.5.2 Ion Dissociation Techniques ""; ""1.5.3 Tandem Quadrupole MS-MS Instruments ""; ""1.5.4 Ion-Trap MS n Instruments ""; ""1.5.5 Tandem TOF (TOF-TOF) Instruments ""

""1.5.6 Hybrid Instruments (Q-TOF, Q-LIT, IT-TOF) """"1.5.7 MS-MS and MSn in FT-ICR-MS ""; ""1.5.8 Orbitrap-Based Hybrid Systems ""; ""1.5.9 Ion-Mobility Spectrometry-Mass Spectrometry ""; ""1.6 Data Interpretation and Analytical Strategies ""; ""1.6.1 Data Acquisition in MS Revisited ""

""1.6.2 Quantitative Bioanalysis and Residue Analysis ""

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on online resource; title from PDF title page (ebrary, viewed February 26, 2015).

ISBN:

9783527673414

3527673415

9783527673421

3527673423

9783527673391

3527673393

OCLC:

903357111