Omics in plant breeding / edited by Aluízio Boreém, Roberto Fritsche-Neto.

Format:

Book

Contributor:

Fritsche-Neto, Roberto, 1983- editor.

Boreém, Aluízio, editor.

Language:

English

Subjects (All):

Plant genetic engineering.

Plant breeding.

Physical Description:

1 online resource (280 p.)

Edition:

1st ed.

Place of Publication:

Ames, Iowa : Wiley Blackwell, 2014.

Language Note:

English

Summary:

The use of computational and high-throughput methods, such as genomics, proteomics, transcriptomics, or known collectively as "-omics", to study plant biology for well over a decade now. As these technologies mature, plant and crop scientists have started using these methods for improving crop varieties. Omics in Plant Breeding provides a timely overview of key omics-based methods and their application in plant breeding. Omics in Plant Breeding is written in a practical and accessible manner, providing the reader with a valuable overview of specific omics-based methods r

Contents:

Cover; Title Page; Copyright; Contents; List of Contributors; Foreword; Chapter 1 Omics: Opening up the ""Black Box"" of the Phenotype; The Post-Genomics Era; The Omics in Plant Breeding; Genomics, Precision Genomics, and RNA Interference; Transcriptomics and Proteomics; Metabolomics and Physiognomics; Phenomics; Bioinformatics; Prospects; References; Chapter 2 Genomics; The Rise of Genomics; DNA Sequencing; Development of Sequence-based Markers; Genetic Mapping; Association Mapping; Genome Wide Selection (GWS); Comparative Genomics; Structural and Comparative Genomics

Applications of Genomics in Plant BreedingReferences; Chapter 3 Transcriptomics; Methods of Studying the Transcriptome; Construction of cDNA Libraries for EST Sequencing; Suppression Subtractive Hybridization (SSH); Serial Analysis of Gene Expression (SAGE); Microarrays; RNA-seq; Applications of Transcriptomics Approaches for Crop Breeding; Conclusions and Future Prospects; Acknowledgements; References; Chapter 4 Proteomics; History; Different Methods for the Extraction of Total Proteins; Importance of High-quality Protein Extracts; Extraction with TCA/Acetone; Phenolic Extraction

Extraction with Phenol and Precipitation with TCA/AcetoneProtein Extraction: Removal of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RuBisCO); Subcellular Proteomics; Post-Translational Modifications; Phosphorylation; Quantitative Proteomics; Quantification with Isotope Labeling of Proteins and Peptides; Label-free Quantification; Perspectives; References; Chapter 5 Metabolomics; Introduction; Metabolomic and Biochemical Molecules; Technologies for Metabolomics; Metabolomic Database Analysis; Metabolomics Applications; Metabolomics-assisted Plant Breeding

Plant Breeding for Quality Metabolic CompoundsMetabolites QTLs (mQTLs) Expression; Simple Studies on the Yield Components; Studies of Multiple Character Production; Associative Genome Mapping and mQTL Profiles; Large-scale Phenotyping Using Metabolomics; Conclusion and Outlook; References; Chapter 6 Physionomics; Introduction; Early Studies on Plant Physiology and the Discovery of Photosynthesis; Biochemical Approaches to Plant Physiology and the Discovery of Plant Hormones; Genetic Approaches to Plant Physiology and the Discovery of Hormone Signal Transduction Pathways

Alternative Genetic Models for Omics Approaches in Plant Physiology""Physionomics"" as an Integrator of Various Omics for Functional Studies and Plant Breeding; Acknowledgements; References; Chapter 7 Phenomics; Introduction; Examples of Large-scale Phenotyping; Important Aspects for Phenomics Implementation; Sampling versus Controlling Environmental Variation; Storage and Data Management; Statistical Analysis of Data; Main Breeding Applications; Germplasm Characterization; Genomic Selection (GS); QTL Identification; Final Considerations; References

Chapter 8 Electrophoresis, Chromatography, and Mass Spectrometry

Notes:

Description based upon print version of record.

Includes bibliographical references at the end of each chapters and index.

Description based on print version record.

ISBN:

9781118820841

1118820843

9781118820971

1118820975

9781118821053

111882105X

OCLC:

881417046