Problem solving in enzyme biocatalysis / Andres Illanes, Lorena Wilson and Carlos Vera.

Format:

Book

Author/Creator:

Illanes, Andrés.

Contributor:

Wilson, Lorena.

Vera, Carlos.

Language:

English

Subjects (All):

Enzymes--Biotechnology.

Enzymes.

Biocatalysis.

Enzyme kinetics.

Enzymes--Industrial applications.

Physical Description:

1 online resource (346 p.)

Edition:

1st ed.

Place of Publication:

Chichester, West Sussex, United Kingdom : Wiley, 2014.

Language Note:

English

Summary:

"The only book to approach enzyme kinetics with a problem-solving focus, for practical applications in the food, pharmaceutical, and fine chemistry industry"-- Provided by publisher.

Contents:

Problem Solving in Enzyme Biocatalysis; Contents; Preface; Nomenclature; Epsilon Software Information; Acknowledgement; 1 Facts and Figures in Enzyme Biocatalysis; 1.1 Introduction; 1.1.1 Enzyme Properties; 1.1.2 Enzyme Applications; 1.2 Enzymes as Process Catalysts; 1.3 Evolution of Enzyme Biocatalysis: From Hydrolysis to Synthesis; 1.4 The Enzyme Market: Figures and Outlook; References; 2 Enzyme Kinetics in a Homogeneous System; 2.1 Introduction; 2.1.1 Concept and Determination of Enzyme Activity; 2.1.2 Definition of a Unit of Activity; 2.1.3 Measurement of Enzyme Activity

2.2 Theory of Enzyme Kinetics2.3 Single-Substrate Reactions; 2.3.1 Kinetics of Enzyme Inhibition; 2.4 Multiple-Substrate Reactions; 2.4.1 Reaction Mechanisms; 2.4.2 Kinetics of Enzyme Reactions with Two Substrates; 2.5 Multiple-Enzyme Reactions; 2.6 Determination of Kinetic Parameters; 2.7 Effects of Operational Variables on Enzyme Kinetics; 2.7.1 Effects of pH; 2.7.2 Effects of Temperature; Solved Problems; Supplementary Problems; References; 3 Enzyme Kinetics in a Heterogeneous System; 3.1 Introduction; 3.2 Immobilization of Enzymes

3.2.1 Immobilization on Solid Supports (Carrier-Bound Systems)3.2.2 Immobilization by Containment; 3.2.3 Immobilization in Carrier-Free Systems; 3.2.4 Parameters of Enzyme Immobilization; 3.2.5 Optimization of Enzyme Immobilization; 3.3 Mass-Transfer Limitations in Enzyme Catalysis; 3.3.1 Partition Effects; 3.3.2 External Diffusional Restrictions in Impervious Biocatalysts; 3.3.3 Internal Diffusional Restrictions in Porous Biocatalysts; 3.4 Determination of Intrinsic Kinetic and Mass-Transfer Parameters; 3.4.1 EDR; 3.4.2 IDR; Solved Problems; Supplementary Problems; References

4 Enzyme Reactor Design and Operation under Ideal Conditions4.1 Modes of Operation and Reactor Configurations; 4.2 Definition of Ideal Conditions; 4.3 Strategy for Reactor Design and Performance Evaluation; 4.4 Mathematical Models for Enzyme Kinetics, Modes of Operation, and Reactor Configurations under Ideal Conditions; 4.4.1 Batch Enzyme Reactor; 4.4.2 Continuous Enzyme Reactors; Solved Problems; Supplementary Problems; References; 5 Enzyme Reactor Design and Operation under Mass-Transfer Limitations; 5.1 Sequential Batch and Continuously Operated Reactors with Immobilized Enzymes

5.2 Mathematical Models for Enzyme Kinetics, Modes of Operation, and Reactor Configurations under Mass-Transfer LimitationsSolved Problems; Supplementary Problems; 6 Enzyme Reactor Design and Operation under Biocatalyst Inactivation; 6.1 Mechanistically Based Mathematical Models of Enzyme Inactivation; 6.2 Effect of Catalytic Modulators on Enzyme Inactivation; 6.3 Mathematical Models for Different Enzyme Kinetics, Modes of Operation, and Reactor Configurations under Biocatalyst Inactivation; 6.3.1 Nonmodulated Enzyme Inactivation; 6.3.2 Modulated Enzyme Inactivation

6.4 Mathematical Models for Enzyme Kinetics, Modes of Operation, and Reactor Configurations under Simultaneous Mass-Transfer Limitations and Enzyme Inactivation

Notes:

Includes index.

Includes bibliographical references and index.

Description based on print version record.

ISBN:

9781118341773

1118341775

9781118341742

1118341740

9781118341759

1118341759

OCLC:

861080316