Advances in medicine and biology Volume 20 / Leon V. Berhardt, editor.

Format:

Book

Contributor:

Berhardt, Leon V.

Series:

Advances in Medicine and Biology

Advances in medicine and biology, 2157-5398 ; v. 20

Language:

English

Subjects (All):

Medicine--Research.

Medicine.

Biology.

Physical Description:

1 online resource (377 p.)

Edition:

1st ed.

Place of Publication:

New York : Nova Science Publishers, Inc., 2011.

Language Note:

English

Summary:

Preface; Troponin T: A Search of Superparamagnetic Iron-Oxide bound Antitroponin Nanoparticle for Magnetic Resonance Imaging; Troponin T: Newer Magnetic Immunoassay Method of Troponins as Point-of-Care Detection of Acute Myocardial Infarction; Genotoxicity Evaluation of Exposure to Lead; Cell Cycle Alteration in Down Syndrome; Alterations of Autonomic Modulation of Heart Rate Variability in Eating Disorders; Antimicrobial-Resistant Gram-Positive Cocci in the Third Millennium: Novel Pharmaceutical Weapons & Their Therapeutic Perspectives; Promising Applications for TLR Agonists as Immune Adjuvants; Current Trends in Glaucoma: What's About Neuroprotection?; Pharmacological Aspects of Borates; Phage Display Technology: New Biotechnological Applications in Synthetic Biology; Analytical Methods for the Quantitative Determination of Oxytocin; Plant Cell Wall Functional Genomics: Novelties from Proteomics; Isolated Rat Hearts Preserved for 24-120 Hours in the Presence of Carbon Monoxide & Carbon Dioxide, Resuscitation & Heterotopic Transplantation; System Specific Chaperones for Membrane Redox Enzyme Maturation in Bacteria; Phenotypic Modification of Staphylococcal Cell Wall Characteristics Mediated by DHEA; Index.

Contents:

Intro

ADVANCES IN MEDICINE AND BIOLOGY. VOLUME 20

CONTENTS

PREFACE

TROPONIN T: A SEARCH OF SUPERPARAMAGNETIC IRON-OXIDE BOUND ANTITROPONIN NANOPARTICLE FOR MAGNETIC RESONANCE IMAGING

Abstract

Introduction

2. Materials and Methods

2.1. Preparation and Characterization of Nonoparticle Complex

2.2. Gadolinium Enhanced T1 Weighted Imaging of Apoferritin-Gadolinium Complex

2.3. Characterization of Cardiac Troponin T/I/C Subtypes and MALDI Imaging

2.4. Development of Antitroponin Based Nanoparticles as MRI Imaging Contrast Agents

Selection of TE and TR for Optimizing Contrast

2.5. MRI Microimaging of Heart and Prototype Tube

2.6. Characterization, Detection of and Measurement of Cardiac Muscle Components

3. Results

3.1. The Polymer Coated Superparamagnetic Biotinylated Antitroponint Microspheres as Image Contrast

3.2. Gadolinium Enhanced T1 Weighted Imaging of Apoferritin-Gadolinium Complex

3.3. Characterization of Cardiac Troponin T/I/C Subtypes and MALDI Imaging

3.4. High Resolution Magnetic Resonance Imaging of Nanoparticles and Post-Injected Mouse Heart

3.5. Characterization, Detection of and Measurement of Cardiac Muscle Components

4. Discussion

5. Limitations and Challenges of the Study

6. The Futuristic View of Art

7. Conclusion

Acknowledgments

References

TROPONIN T: NEWER MAGNETIC IMMUNOASSAY METHOD OF TROPONINS AS POINT-OF-CARE DETECTION OF ACUTE MYOCARDIAL INFARCTION

1. Introduction

1.2. Synthesis of Nanomagnetic Particles for Biomedical Applications

1.3. Characterization of Antitroponin Based Nanoparticle Complex by Sandwich ELISA

1.4. Principle of Nanoparticle in Magnetic Assay

2. AMI Detectable Device

2.1. The POCM Device.

2.2. Device for Acute Myocardial Infarction Detection and Troponin Screening

2.3. Principle of Dry Chemistry Glucodetection in Troponin Estimation

2.4. Calibration of Glucose Measurement

2.2. The Use of the Fluorescent Dry Chemistry in Glucodetection Device

3. Detection of Troponin T as Point-of-Care AMI Test

4. Approach of Troponin MALDI Analysis

5. MALDI-TOF Analysis in AMI Serum

6. Limitations

Conclusion

GENOTOXICITY EVALUATION OF EXPOSURE TO LEAD

ABSTRACT

1. INTRODUCTION

1.1. Lead Modes of Action

Interaction with Sulphydryl Groups of Proteins

Supplanting of Essential Polyvalent Cations

Free Radical Production

1.2. Genotoxicity Tests

2. HYPOXANTHINE-GUANINE PHOSPHORIBOSYL- TRANSFERASE GENE MUTATION ASSAY

3. T CELL RECEPTOR MUTATION ASSAY

4. CHROMOSOME ABERRATIONS

4.1. In Vitro Studies

4.2. In Vivo Studies

4.3. Epidemiological Studies

5. SISTER CHROMATID EXCHANGES

5.1. In Vitro Studies

5.2. In Vivo Studies

5.3. Epidemiological Studies

6. MICRONUCLEUS TEST

6.1. In Vitro Studies

6.2. In Vivo Studies

6.3. Epidemiological Studies

7. SINGLE CELL GEL ELECTROPHORESIS (COMET) ASSAY

7.1. In Vitro Studies

7.2. In Vivo Studies

7.3. Epidemiological Studies

CONCLUDING REMARKS

ACKNOWLEDGMENTS

REFERENCES

CELL CYCLE ALTERATION IN DOWN SYNDROME

INTRODUCTION

CELL CYCLE AND NEUROGENESIS ALTERATIONS IN DOWN SYNDROME

CONTRIBUTION OF CHROMOSOME 21 GENES IN TUMORS AND CANCERS

Single Minded Gene (SIM2)

T-Cell Lymphoma Invasion and Metastasis 1 Gene, TIAM1

CONTRIBUTION OF CHROMOSOME 21 GENES IN LEUKEMIA

Cooperation of Chromosome 21 Genes with GATA1 During Leukemogenesis

Acute Myeloid Leukaemia Gene, AML1

ETS Transcription Factors ETS2 and ERG.

Basic Leucine Zipper Transcription Factor 1, BACH1

CONCLUSIONS

ALTERATIONS OF AUTONOMIC MODULATION OF HEART RATE VARIABILITY IN EATING DISORDERS

HRV ANALYSIS: METHODOLOGICAL AND GENERAL ASPECTS

Heart Rate Variability in Eating Disorders

ENDOCRINE CHANGES AND HRV IN EATING DISORDERS: A PERSPECTIVE FOR FUTURE INVESTIGATIONS

ANTIMICROBIAL-RESISTANT GRAM-POSITIVE COCCI IN THE THIRD MILLENNIUM: NOVEL PHARMACEUTICAL WEAPONS AND THEIR THERAPEUTIC PERSPECTIVES

EPIDEMIOLOGICAL EXPERIENCE AT A MAJOR TERTIARY CARE HOSPITAL IN NORTHERN ITALY

NOVEL ANTIBACTERIAL AGENTS WITH ENHANCED ACTIVITY AGAINST RESISTANT GRAM POSITIVE COCCI

Quinupristin/Dalfopristin

Linezolid

Daptomycin

Tigecycline

Dalbavancin

Oritavancin

Telavancin

CLINICAL INDICATIONS OF NOVEL ANTIBIOTICS WITH EXPANDED SPECTRUM AGAINST RESISTANT GRAM-POSITIVE COCCI

Skin and Soft-Tissue Infections

Bone and Joint Infections

Pneumonia and Lower Respiratory Tract Infections

Intra-abdominal Infections

Bacteremia and Endocarditis

POTENTIAL SYNERGISTIC INTERACTIONS OF NEWER ANTIBIOTICS: IN VITRO STUDIES

PROMISING APPLICATIONS FOR TLR AGONISTS AS IMMUNE ADJUVANTS

Untitled

STRUCTURE

Signaling: TLR Activation

MyD88-Dependent Pathway

MyD88-Independent (TRIF-Dependent) Pathway

TLR Agonists as Adjuvants

How Do TLR Agonists Stimulate Adaptive Immunity?

Cross-Priming of CD8 T Cells

IL-12 and Th1 Responses

Reversal of Tolerance

Upregulation of Co-Stimulatory Molecules

TLR AGONISTS WITH POTENTIAL CLINICAL USE

TLR2

TLR4

TLR5

TLR7/8

TLR9

CONCLUSION

CURRENT TRENDS IN GLAUCOMA: WHAT'S ABOUT NEUROPROTECTION?

INTRODUCTION.

1. Epidemiology And Risk Factors In Glaucoma

2. Biphasic Theory For Glaucoma Damage

2.1. Mechanical and vascular factors trigger glaucoma pathogenesis

2.2. Secondary degeneration

a) Molecular changes

Neurotrophin deprivation

Activation of the caspase enzyme family

Glutamate induced excitotoxicity

ATP releases

TNF-α

Oxidative stress and free radicals

Increased accumulation of self-proteins

Increased expression of pro-apoptotic genes

b) Cellular changes

"Activation" response of glial cells

Muller cell gliosis

Astrocyte induced vasoconstriction

3.Glaucoma And Neuroprotection

3.1. Neuroprotective Drugs In Glaucoma

3.1.1.Ocular hypotensive drugs

a). Antiglaucomatous agents

b). Cannabinoids

3.2. Other drugs

a). Tetracyclines

Minocycline

Doxycycline

b). Ca 2+ channel blockers

Nifedipine

Flunarizine

c). Antioxidants

d) NMDA antagonists, p38 MAPK, and caspase inhibitors

e). 17beta-estradiol

f). Nitric oxide synthase (NOS-2) inhibitors (Aminoguanidine)

g). Erythropoietin

4. Future Neuroprotective Therapies For Glaucoma

4.1. Neurotrophins

4.2. Controlling Infamation: Anti-TNF-α-Strategies

4.3 Inmunomodulatory Compounds: Glatiramer Acetate(GA)

4.4 Gene Therapy

4.5 siRNA

4.6 Stem Cells

PHARMACOLOGICAL ASPECTS OF BORATES

2. CLINICAL USES

2.1. Antibacterial Agent

2.2. Antifungal Agent

2.3. Chemopreventive Agent for Human Cancer

2.4. Wound Treatment

2.5. Ear Infections

2.6. Dental Treatment

3. TOXICITY

3.1. Human Toxicity

3.2. Embryotoxicity

3.3. Developmental and Reproductive Toxicity

3.4. Genotoxicity

3.5. Phytotoxicity

4. ABSORPTION OF BORIC ACID

5. DISSOLUTION KINETICS

6. PHARMACOKINETICS OF BORIC ACID

8. OXIDATIVE STRESS.

9. ENZYME INHIBITION

10. SYNTHESIS OF BIOACTIVE COMPOUNDS

11. ANALYSIS IN BIOLOGICAL FLUIDS

12. PRESERVATION AND INSECT CONTROL

12.1. Preservative

12.2. Insecticide

PHAGE DISPLAY TECHNOLOGY: NEW BIOTECHNOLOGICAL APPLICATIONS IN SYNTHETIC BIOLOGY

SYNTHETIC BIOLOGY AND THE "NEVER BORN PROTEINS"

PROTEIN ENGINEERING AND DIRECTED EVOLUTION

IN VITRO AND BIOLOGICAL DISPLAY TECHNOLOGIES

PHAGE DISPLAY FOR DIRECTED MOLECULAR EVOLUTION

ANTIBODY PHAGE

PHAGE AS PROBES IN NANOBIOTECHNOLOGY

VIRUSES AS NEW MATERIALS

PHAGE PERSPECTIVES IN SYNTHETIC BIOLOGY

ANALYTICAL METHODS FOR THE QUANTITATIVE DETERMINATION OF OXYTOCIN

BIOASSAY

PARTITION AND THIN LAYER CHROMATOGRAPHY

ELECTROPHORETIC SEPARATIONS

REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

UV DETECTION

FLUORESCENCE METHODS WITH DERIVATIZATION

PHOTODIODE ARRAY DETECTION

COULOMETRIC DETERMINATION

LC MS METHODS

CATION EXCHANGE CHROMATOGRAPHY

RADIO-IMMUNOASSAY

ENZYME-LINKED IMMUNO-SORBENT ASSAY

PLANT CELL WALL FUNCTIONAL GENOMICS: NOVELTIES FROM PROTEOMICS

IS PROTEOMICS REDUNDANT WITH REGARD TO TRANSCRIPTOMICS?

A SUMMARY OF PRESENT PROTEOMIC RESULTS OBTAINED ON ARABIDOPSIS THALIANA

WHY INVESTIGATING ADDITIONAL CELL WALL PROTEOMES?

ISOLATED RAT HEARTS PRESERVED FOR 24-120 HOURS IN THE PRESENCE OF ARBON MONOXIDE AND CARBON DIOXIDE, RESUSCITATION AND HETEROTOPIC TRANSPLANTATION

SECTION 1: DESICCATION AND PRESERVATION WITH CARBON DIOXIDE

SECTION 2: DESICCATION AND PRESERVATION WITH CARBON MONOXIDE.

SECTION 3: DESICCATION AND PRESERVATION WITH CARBON MONOXIDE, CARBON DIOXIDE, HELIUM AND OXYGEN.

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-62100-056-7

OCLC:

847478931

Publisher Number:

9781612091358