Metabolomics in practice : successful strategies to generate and analyze metabolic data / edited by Michael Lämmerhofer and Wolfram Weckwerth.

Format:

Book

Contributor:

Lämmerhofer, Michael.

Weckwerth, Wolfram.

Language:

English

Subjects (All):

Metabolism.

Metabolism--Research--Methodology.

Physical Description:

1 online resource (443 p.)

Edition:

1st ed.

Place of Publication:

Weinheim an der Bergstrasse, Germany : Wiley-VCH Verlag GmbH, c2013.

Language Note:

English

Summary:

Unlike other handbooks in this emerging field, this guide focuses on the challenges and critical parameters in running a metabolomics study, including such often-neglected issues as sample preparation, choice of separation and detection method, recording and evaluating data as well as method validation. By systematically covering the entire workflow, from sample preparation to data processing, the insight and advice offered here helps to clear the hurdles in setting up and running a successful analysis, resulting in high-quality data from every experiment.Based on more than a decade of pra

Contents:

Metabolomics in Practice; Contents; List of Contributors; Preface; 1 The Sampling and Sample Preparation Problem in Microbial Metabolomics; 1.1 Introduction; 1.2 Microorganisms and Their Properties; 1.3 Sampling Methods; 1.3.1 The Need for Rapid Sampling; 1.3.2 Sampling Systems; 1.4 Quenching; 1.4.1 Quenching Procedures and Their Properties; 1.4.2 Validation of the Quenching Procedure and Minimization of Metabolite Leakage; 1.4.3 Quenching Procedure for Determination of Intracellular Metabolites in the Presence of Extracellular Abundance; 1.4.4 Quenching of Bacteria; 1.5 Metabolite Extraction

1.5.1 Extraction Methods and Their Properties1.5.2 Validation of Extraction Methods for Yeast Metabolomics; 1.6 Application of 13C-Labeled Internal Standards; 1.7 Conclusions; References; 2 Tandem Mass Spectrometry Hyphenated with HPLC and UHPLC for Targeted Metabolomics; 2.1 Introduction; 2.2 LC-MS-Based Targeted Metabolomics; 2.3 Liquid Chromatography; 2.4 Mass Spectrometry; 2.4.1 Ionization Techniques; 2.4.2 Mass Analyzers; 2.5 Sample Preparation; 2.6 Relative and Absolute Quantification; 2.7 Applications; 2.8 Synopsis; References; 3 Uncertainty of Measurement in Quantitative Metabolomics

3.1 Introduction3.1.1 MS-Based Techniques in Metabolomics; 3.1.2 Uncertainty of Measurement in Quantitative Analysis; 3.1.2.1 Definition; 3.1.2.2 Uncertainty Calculation According to the Bottom-Up Approach; 3.2 Uncertainties of Quantitative MS Experiments; 3.2.1 Uncertainties in Sample Preparation; 3.2.1.1 Sampling and Sample Preparation in Metabolite Profiling in Fermentations; 3.2.1.2 Calculation of Sample Preparation Uncertainty for Intracellular Metabolite Quantitation in Yeast: A Practical Example; 3.2.1.3 LC-MS

3.2.2 Uncertainty of Mass Spectrometric Assays (LC-MS and GC-MS Measurements)3.2.2.1 GC-MS; 3.2.2.2 Calculation of Uncertainty for LC-MS Measurements of Cell Extracts: A Practical Example; 3.3 Concluding Remarks; Abbreviations; Acknowledgment; References; 4 Gas Chromatography and Comprehensive Two-Dimensional Gas Chromatography Hyphenated with Mass Spectrometry for Targeted and Nontargeted Metabolomics; 4.1 Introduction and Scope; 4.2 Sample Preparation for GC-Based Metabolite Profiling; 4.3 GC-MS and GC x GC-TOFMS Instrumentation for Metabolomics; 4.4 Data Analysis Strategies and Software

4.5 Illustrative Examples and Concluding RemarksReferences; 5 LC-MS-Based Nontargeted Metabolomics; 5.1 Introduction; 5.2 LC-MS-Based Untargeted Metabolomics; 5.2.1 LC Issues; 5.2.2 Mass Spectrometry; 5.3 Study Design; 5.4 Sample Preparation; 5.5 Analytical Strategies; 5.6 Data Analysis; 5.7 Metabolite Identification; 5.8 Applications; 5.9 Synopsis; References; 6 The Potential of Ultrahigh Resolution MS (FTICR-MS) in Metabolomics; 6.1 Introduction; 6.2 Metabolomics Technologies; 6.3 Principles of FTICR-MS; 6.3.1 Natural Ion Movement Inside an ICR Cell Subjected to Magnetic and Electric Fields

6.3.2 Applied Physical Techniques in FTICR-MS

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on online resource; title from title page (ebrary, viewed April 11, 2013).

ISBN:

9783527655861

3527655867

9781299313545

129931354X

9783527655892

3527655891

9783527655885

3527655883

OCLC:

829460567