Plant tissue culture : techniques and experiments / Roberta H. Smith.

Format:

Book

Author/Creator:

Smith, Roberta H.

Language:

English

Subjects (All):

Plant tissue culture--Laboratory manuals.

Plant tissue culture.

Tissue culture.

Physical Description:

1 online resource (205 p.)

Edition:

3rd ed.

Place of Publication:

Amsterdam ; Boston : Elsevier/AP, 2012.

Language Note:

English

Summary:

Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experien

Contents:

Front Cover; Plant Tissue Culture: Techniques and Experiments; Coyright; Contents; Preface; Scheduling and Interrelationships of Exercises; Chapter 1 - History of Plant Cell Culture; INTRODUCTION; THE EARLY YEARS; THE ERA OF TECHNIQUES DEVELOPMENT; THE RECENT PAST; THE PRESENT ERA; REFERENCES; Chapter 2 - Setup of a Tissue Culture Laboratory; WORK AREAS; Chapter 3 - Media Components and Preparation; INORGANIC SALTS; PLANT GROWTH REGULATORS; VITAMINS; CARBOHYDRATES; HEXITOLS; GELLING AGENT; AMINO ACIDS; ANTIBIOTICS; NATURAL COMPLEXES; MEDIA PH; MEDIUM PREPARATION; REFERENCES

Chapter 4 - Explant PreparationEXPLANT AGE; SEASON; EXPLANT SIZE; PLANT QUALITY; GOAL; GENOTYPE; ASEPTIC TECHNIQUE; ASEPTIC GERMINATION OF SEEDS; Chapter 5 - Contamination; EXPLANT; PERSONNEL; LAMINAR AIR FLOW HOOD; INSECTS; MEDIA; INSTRUMENTS; ROOM AIR HANDLING SYSTEM; REFERENCES; Chapter 6 - Callus Induction; CALLUS INITIATION; EXPLANT ORIENTATION; ESTABLISHMENT OF COMPETENT CEREAL CELL CULTURES; SALT SELECTION IN VITRO; GROWTH CURVES; CELLULAR VARIATION FROM CALLUS CULTURES; BIBLIOGRAPHY; Chapter 7 - Regeneration and Morphogenesis; CONTROLLED MORPHOGENESIS: POTATO TUBERIZATION

SOMATIC EMBRYOGENESISREGENERATION OF RICE; DORMANCY REQUIREMENTS OF EXPLANTS; PRIMARY MORPHOGENESIS: DOUGLAS FIR; BIBLIOGRAPHY; Chapter 8 - Woody Shrubs and Trees; RHODODENDRONS AND AZALEAS; ROSES; BIRCH TREES; WHITE CEDAR (ARBORVITAE); REFERENCES; Chapter 9 - Haploid Plants from Anther Culture; DATURA ANTHER CULTURE; AFRICAN VIOLET; TOBACCO; ANTHER SQUASH TECHNIQUE; BIBLIOGRAPHY; Chapter 10 - Embryo Rescue; SWEET CORN EMBRYO CULTURE; CRABAPPLE AND PEAR; BIBLIOGRAPHY; Chapter 11 - Meristem Culture for Virus-Free Plants; ISOLATION OF THE SHOOT APICAL MERISTEM

ESTABLISHING VIRUS- AND BACTERIA-FREE PLANTSGARLIC PROPAGATION FROM BULB SCALES; BIBLIOGRAPHY; Chapter 12 - In Vitro Propagation for Commercial Production of Ornamentals; BOSTON FERN; STAGHORN FERN; FICUS; KALANCHOE; AFRICAN VIOLET; ENDANGERED SPECIES; CACTUS; BIBLIOGRAPHY; Chapter 13 - Protoplast Isolation and Fusion; PLANT PROTOPLAST ISOLATION AND FUSION; Chapter 14 - Agrobacterium-Mediated Transformation of Plants; PETUNIA OR TOBACCO LEAF DISK; PETUNIA SHOOT APEX; TOBACCO LEAF INFILTRATION; Appendix I - Useful Measurements; Appendix II - Review of Solution Preparation

Appendix III - List of SuppliersAppendix IV - Common Plant Tissue Culture Terms1; Index

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

9786613784988

9781281604293

1281604291

9780124159853

0124159850

OCLC:

804664680