Topics in fluorescence spectroscopy: principles / edited by Joseph R. Lakowicz.

Format:

Book

Contributor:

Lakowicz, Joseph R.

Series:

Topics in fluorescence spectroscopy ; v.2.

Topics in fluorescence spectroscopy ; v.2

Language:

English

Subjects (All):

Diagnostic imaging.

Fluorescence spectroscopy.

Lasers--Diagnostic use.

Lasers.

Physical Description:

1 online resource (449 p.)

Edition:

1st ed. 2002.

Place of Publication:

New York : Kluwer Academic, c2002.

Language Note:

English

Summary:

Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis, and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of mac- molecules, to probe the structures of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry. Because of these numerous developments and the rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial two volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy. The third volume will appear shortly. The first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3).

Contents:

Fluorescence Anisotropy: Theory and Applications

Fluorescence Quenching: Theory and Applications

Resonance Energy Transfer

Least-Squares Analysis of Fluorescence Data

The Global Analysis of Fluorescence Intensity and Anisotropy Decay Data: Second-Generation Theory and Programs

Fluorescence Polarization from Oriented Systems

Fluorescence-Based Fiber-Optic Sensors

Inhomogeneous Broadening of Electronic Spectra of Dye Molecules in Solutions.

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-280-20633-0

9786610206339

0-306-47058-6

OCLC:

923695963