Microcalorimetry of macromolecules : the physical basis of biological structures / Peter L. Privalov.

Format:

Book

Author/Creator:

Privalov, Peter L.

Series:

Wiley series in protein and peptide science Microcalorimetry of macromolecules

Language:

English

Subjects (All):

Macromolecules.

Macromolecules--Analysis.

Calorimeters.

Physical Description:

1 online resource (422 p.)

Edition:

1st ed.

Place of Publication:

Hoboken, N.J. : Wiley, 2012.

Language Note:

English

Summary:

"This is the first textbook on the microcalorimetry of biological molecules. The coverage starts from the basics of thermodynamics (which are unknown for many scientists working in biology), describes evolution of the calorimetric technique, explains how to analyze the calorimetric data, and illustrates these methods with a wide selection of examples. The book provides an essential resource to scientists studying biological molecular structures and the reactions between these structures"-- Provided by publisher.

Contents:

MICROCALORIMETRY OF MACROMOLECULES; CONTENTS; 1: INTRODUCTION; 2: METHODOLOGY; 2.1. THERMODYNAMIC BASICS OF CALORIMETRY; 2.1.1. Energy; 2.1.2. Enthalpy; 2.1.3. Temperature; 2.1.4. Energy Units; 2.1.5. Heat Capacity; 2.1.6. Kirchhoff's Relation; 2.1.7. Entropy; 2.1.8. Gibbs Free Energy; 2.2. EQUILIBRIUM ANALYSIS; 2.2.1. Two-State Transition; 2.2.2. Derivatives of the Equilibrium Constant; 2.3. AQUEOUS SOLUTIONS; 2.3.1. Specificity of Water as a Solvent; 2.3.2. Acid-Base Equilibrium; 2.3.3. Partial Quantities; 2.4. TRANSFER OF SOLUTES INTO THE AQUEOUS PHASE; 2.4.1. Hydration Effects

2.4.2. Hydrophobic Force2.4.3. Hydration of Polar and Nonpolar Groups; REFERENCES; 3: CALORIMETRY; 3.1. ISOTHERMAL REACTION MICROCALORIMETRY; 3.1.1. The Heat of Mixing Reaction; 3.1.2. Mixing of Reagents in Comparable Volumes; 3.1.3. Isothermal Titration Microcalorimeter; 3.1.4. ITC Experiments; 3.1.5. Analysis of the ITC Data; 3.2. HEAT CAPACITY CALORIMETRY; 3.2.1. Technical Problems; 3.2.2. Differential Scanning Microcalorimeter; 3.2.3. Determination of the Partial Heat Capacity of Solute Molecules; 3.2.4. DSC Experiments

3.2.5. Determination of the Enthalpy of a Temperature-Induced Process3.2.6. Determination of the van't Hoff Enthalpy; 3.2.7. Multimolecular Two-State Transition; 3.2.8. Analysis of the Complex Heat Capacity Profile; 3.2.9. Correction for Components Refolding; 3.3. PRESSURE PERTURBATION CALORIMETRY; 3.3.1. Heat Effect of Changing Pressure; 3.3.2. Pressure Perturbation Experiment; REFERENCES; 4: MACROMOLECULES; 4.1. EVOLUTION OF THE CONCEPT; 4.2. PROTEINS; 4.2.1. Chemical Structure; 4.2.2. Physical Structure; 4.2.3. Restrictions on the Conformation of Polypeptide Chains

4.2.4. Regular Conformations of Polypeptide Chain Proteins4.3. HIERARCHY IN PROTEIN STRUCTURE; 4.3.1. Tertiary Structure of Proteins; 4.3.2. Quaternary Structure of Proteins; 4.4. NUCLEIC ACIDS; 4.4.1. Chemical Structure; 4.4.2. Physical Structure; REFERENCES; 5: THE α-HELIX AND α-HELICAL COILED-COIL; 5.1. THE α-HELIX; 5.1.1. Calorimetric Studies of α-Helix Unfolding-Refolding; 5.1.2. Analysis of the Heat Capacity Function; 5.2. α-HELICAL COILED-COILS; 5.2.1. Two-Stranded Coiled-Coils; 5.2.2. Three-Stranded Coiled-Coils; 5.3. α-HELICAL COILED-COIL PROTEINS; 5.3.1. Muscle Proteins

5.3.2. Myosin Rod5.3.3. Paramyosin; 5.3.4. Tropomyosin; 5.3.5. Leucine Zipper; 5.3.6. Discreteness of the Coiled-Coils; REFERENCES; 6: POLYPROLINE-II COILED-COILS; 6.1. COLLAGENS; 6.1.1. Collagen Superhelix; 6.1.2. Hydrogen Bonds in Collagen; 6.1.3. Stability of Collagens; 6.1.4. Role of Pyrrolidine Rings in Collagen Stabilization; 6.2. CALORIMETRIC STUDIES OF COLLAGENS; 6.2.1. Enthalpy and Entropy of Collagen Melting; 6.2.2. Correlation between Thermodynamic and Structural Characteristics of Collagens; 6.2.3. Role of Water in Maintaining the Collagen Structure

6.3. THERMODYNAMICS OF COLLAGENS

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

9786613662453

9781280685514

1280685514

9781118337493

1118337492

9781118337509

1118337506

9781118337479

1118337476

OCLC:

793814212