Transcriptional regulation in eukaryotes : concepts, strategies, and techniques / Michael Carey, Stephen T. Smale.

Format:

Book

Author/Creator:

Carey, Michael (Michael F.)

Contributor:

Smale, Stephen T.

Language:

English

Subjects (All):

Genetic transcription--Regulation.

Genetic transcription.

Transcription factors.

Genetic transcription--Regulation--Research--Methodology.

Physical Description:

1 online resource (670 p.)

Place of Publication:

Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press, c2000.

Language Note:

English

Summary:

In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene and the biochemistry of a new transcription factor. This important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states.

Contents:

Intro

Contents

Preface

Overview

Abbreviations and Acronyms

A Primer on Transcriptional Regulation in Mammalian Cells

Initial Strategic Issues

Modes of Regulating mRNA Abundance

Transcription Initiation Site Mapping

Functional Assays for Promoter Analysis

Identification and Analysis of Distant Control Regions

Identifying cis-Acting DNA Elements within a Control Region

Identification of DNA-binding Proteins and Isolation of Their Genes

Confirming the Functional Importance of a ProteinÒ DNA Interaction

In Vivo Analysis of an Endogenous Control Region

Approaches for the Synthesis of Recombinant Transcription Factors

Identifying and Characterizing Transcription Factor Domains

Theory, Characterization, and Modeling of DNA Binding by Regulatory Transcription Factors

Crude and Fractionated Systems for In Vitro Transcription

Approaches for Studying Transcription Complex Assembly

Cautions

Suppliers

Trademarks

Index.

Notes:

Bibliographic Level Mode of Issuance: Monograph

Includes bibliographical references and index.

ISBN:

0-87969-665-6

OCLC:

51217367