Microbial conversions of raw glycerol / George Aggelis, editor.

Format:

Book

Contributor:

Aggelis, George.

Language:

English

Subjects (All):

Glycerin--Biotechnology.

Glycerin.

Microbial biotechnology.

Industrial microbiology.

Physical Description:

1 online resource (209 p.)

Edition:

1st ed.

Place of Publication:

New York : Nova Biomedical Books, c2009.

Language Note:

English

Summary:

In this text, the biochemical pathways of glycerol metabolism in prokaryotic and eukaryotic cells cultivated under various conditions are discussed.

Contents:

Intro

MICROBIAL CONVERSIONS OF RAW GLYCEROL

Contents

Preface

Chapter I Glycerol Waste from Biodiesel Manufacturing

Abstract

1. Introduction

2. Current Status of Biodiesel Production

3. How Biodiesel is Made

4. Characterizations of Glycerol Waste

5. Utilization of Glycerol Waste

6. Conclusion

References

Chapter II Pathways to Aerobic Glycerol Catabolism and their Regulation

Introduction

Glycerol Transport

Glycerol Catabolism

Regulation of Glycerol Assimilation

Genes Implicated in Glycerol Assimilation and their Regulation

Conclusion

Chapter III Citric Acid Production from Raw Glycerol by Yarrowia Lipolytica Wratislavia 1.31

Materials and Methods

Microorganism

Media and Culture Conditions

Analytical Methods

Results and Discussion

Acknowledgement

Chapter IV Biodiesel By-Products Used as Substrates for Oxalic Acid Production by Aspergillus Niger

Conclusions

Chapter V Production of Omega-3 Polyunsaturated Fatty Acids from Biodiesel-derived Crude Glycerol by Microalgal and Fungal Fermentation

1.1. Structure and Significance of ω-3 PUFAs

1.2. Biosynthesis of ω-3 PUFAs

1.3. Sources of ω-3 PUFAs

1.4. Microbial Production of Omega-3 Fatty Acids

2. Producing DHA from Microalgae Grown on Crude Glycerol

2.1. Experimental Protocols

2.1.1. Algal Strain, Medium and Culture Conditions

2.1.2. Crude Glycerol Characterization and Pretreatment

2.1.3. Analysis

2.2. Feasibility of Producing DHA from Crude Glycerol

2.3. Effects of Glycerol Impurities on Algal DHA Production.

2.3.1. Effects of Methanol

2.3.2. Effects of Soap

2.4. Optimization of Algal Culture Conditions for Producing DHA from Crude Glycerol

3. Producing EPA from Fungi Grown on Crude Glycerol

3.1. Experimental Protocols

3.1.1. Fungal Strain and Culture Conditions

3.1.2. Crude Glycerol Characterization and Pretreatment

3.1.3. Analysis

3.2. Feasibility of Producing EPA from Crude Glycerol

3.3. Optimization of Fungal Culture Conditions for Producing EPA from Crude Glycerol

4. Prospects for Producing DHA/EPA Using Crude Glycerol

5. Conclusion

Chapter VI Biotechnological Production of Bioplastics from Raw Glycerol

Occurrence of PHAs

Diversity of PHAs

Applications and Properties of PHAs

Degradation of PHAs

Pathway and Regulation of scl PHA Synthesis

Fermentative Production of PHAs from Crude Glycerol

Recovery of the PHAs

Acknowledgments

Chapter VII Single Cell Oil and Gamma-linolenic Acid Production by Thamnidium elegans Grown on Raw Glycerol

Notation

Microorganisms and Culture Conditions

Fungal Lipid Content Determination

Fractionation of Fungal Lipids

Gas Chromatography (GC) Analysis

Results

Screening of Oleaginous Zygomycetes on Raw Glycerol

Model Presentation

Model Fitted on the Experimental Data and Evaluation of Parameters

Growth of T. elegans on Raw Glycerol

Lipid Composition during Growth of T. elegans

Fatty Acid Composition of the Produced Lipids

Discussion

Chapter VIII The Potential of Raw Glycerol in the Production of Food Grade Carotenoids by Fungi

Abbreviations Used

Fungal Carotenoids.

Food-grade Carotenoids by Blakeslea Trispora

Biosynthesis of Carotenoids

Carotenoid Production in Various Medium Formulations

Carbon Sources

Sugars and other Pure Carbon Sources

Waste-based Media

Nitrogen Sources

Carbon-to-Nitrogen Ratio

Performance of Pure Glycerol as a Carbon Source for Fungal Growth and Carotenoid Production

Using Pure Glycerol for Growth and Carotenoid Production by Blakeslea Trispora

Glycerol as a Sole Carbon Source

Glycerol as co-Substrate of Glucose

Using Pure Glycerol for Growth and Carotenoid Production by other Fungi

Potential of Raw Glycerol in Fungal Carotenoid Production

Safety Aspects

Acknowledgment

Reference List

Chapter IX Characterization of Microbial Biomass Production from Glycerin Waste by Various Yeast Strains

Material and Methods

Reference

Chapter X Microbial Conversion of Glycerol into 1,3-propanediol: Glycerol Assimilation, Biochemical Events Related with 1,3-Propanediol Biosynthesis and Biochemical Engineering of the Process

2. The Biochemistry of 1,3-propanediol Fermentation

2.1. Assimilation of Glycerol inside the Microbial Prokaryotic Cell

2.2. Biochemical Events Related with Glycerol Breakdown in Glycerol-consuming Prokaryotic Microorganisms

3. The Process of 1,3-propanediol Fermentation

4. Conclusions

Chapter XI Capital and Manufacturing Cost Estimation of a Bioprocess Converting Raw Glycerol to 1,3-Propanediol

2. Biotechnological Conversion of Raw Glycerol to 1,3-Propanediol

3. Methodology for Manufacturing Cost Estimation

4. Process Flow Diagram Description

5. Equipment Cost Estimation

6. Estimation of the Operating Costs and Cost of Manufacture.

7. Conclusion

Index

Blank Page.

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on print version record and CIP data provided by publisher.

ISBN:

1-61728-015-1

OCLC:

923662881