Practical cell analysis / Dimitri Pappas.

Format:

Book

Author/Creator:

Pappas, Dimitri.

Language:

English

Subjects (All):

Cytology--Technique.

Cytology.

Biology.

Physical Description:

1 online resource (316 p.)

Edition:

2nd ed.

Place of Publication:

Hoboken, N.J. : Wiley, 2010.

Language Note:

English

Summary:

As analytical chemistry and biology move closer together, biologists are performing increasingly sophisticated analytical techniques on cells. Chemists are also turning to cells as a relevant and important sample to study newly developed methods. Practical Cell Analysis provides techniques, hints, and time-saving tips explaining what may be "common knowledge" to one field but are often hidden or unknown to another. Within this practical guide: The procedures and protocols for cell separation, handling cells on a microscope and for using cells in microfluidic

Contents:

Practical Cell Analysis; Contents; Preface; Acknowledgments; 1 Getting Started (and Getting the Cells); 1.1 INTRODUCTION; 1.2 THE DRIVING NEED; 1.3 PRIMARY AND CULTURED CELLS; 1.4 CHOOSING A CULTURED CELL; 1.5 CHOOSING PRIMARY CELLS; 1.6 EASILY OBTAINABLE PRIMARY CELLS; 1.7 PRIMARY CELLS FROM TISSUES; 1.8 PURIFYING PRIMARY CELLS; 1.9 HOW LONG DO PRIMARY CELLS REMAIN PRIMARY?; 1.10 OBTAINING PRIMARY CELLS FROM A COMMERCIAL SOURCE; 1.11 BACTERIA AND YEAST; 1.12 PRACTICAL ASPECTS OF CELL CULTURE; 1.13 SAFETY ASPECTS OF PRIMARY AND TRANSFORMED CELL LINES

1.14 TRANSFECTION OF PRIMARY AND TRANSFORMED CELL LINES1.15 CONCLUSION; REFERENCES; 2 The Cell-Culture Laboratory (Tools of the Trade); 2.1 INTRODUCTION; 2.2 ISSUES CONCERNING A CELL LABORATORY; 2.3 SETTING UP A CELL CULTURE LABORATORY; 2.4 CELL LINE STORAGE; 2.5 PERSONAL PROTECTIVE EQUIPMENT; 2.6 CELL AND SAMPLE HANDLING; 2.7 COMMON ANALYTICAL INSTRUMENTATION FOR CELL CULTURE; 2.8 CONSIDERATIONS WHEN SETTING UP A CELL-CULTURE LABORATORY; 2.9 ESTABLISHING AND REGULATING A CULTURE FACILITY; 2.10 CONCLUSION; REFERENCES; 3 Maintaining Cultures; 3.1 INTRODUCTION; 3.2 MEDIUM

3.3 THE USE OF MEDIUM IN ANALYSIS, AND ALTERNATIVES3.4 CULTURING CELLS; 3.5 GROWING CELLS IN THREE DIMENSIONS; 3.6 STERILITY AND CONTAMINATION OF CULTURE; 3.7 STORAGE OF CELL SAMPLES AND CELL LINES; PROTOCOL 3.2: CRYOPRESERVATION OF MAMMALIAN CELLS; PROTOCOL 3.3: RETRIEVAL OF CELLS FROM LIQUID-NITROGEN STORAGE; 3.8 CONCLUSION; REFERENCES; 4 Microscopy of Cells; 4.1 INTRODUCTION; 4.2 MICROSCOPE TYPES; 4.3 CULTURING CELLS FOR MICROSCOPY; 4.4 SIGNALS, BACKGROUND, AND ARTIFACTS IN OPTICAL MICROSCOPY; 4.5 STAINING CELLS FOR FLUORESCENCE MICROSCOPY

PROTOCOL 4.1 FIXATION OF CELLS FOR IMMUNOCHEMICAL STAINING4.6 MULTIPLE LABELS; 4.7 VIABILITY AND TWO-PHOTON MICROSCOPY; 4.8 SPATIAL RESOLUTION IN OPTICAL MICROSCOPY; 4.9 IMAGE SATURATION AND INTENSITY; 4.10 ATOMIC FORCE AND ENVIRONMENTAL SCANNING ELECTRON MICROSCOPY; 4.11 CONCLUSION; REFERENCES; 5 Separating Cells; 5.1 INTRODUCTION; 5.2 THE CELL SAMPLE; 5.3 LABEL-FREE (INTRINSIC) SEPARATIONS; 5.4 IMMUNOMAGNETIC SORTING; 5.5 CELL-AFFINITY CHROMATOGRAPHY; 5.6 AFFINITY CHEMISTRY CONSIDERATIONS IN CAC AND MACS SEPARATIONS; PROTOCOL 5.1: SCREENING OF ANTIBODY CLONES

5.7 ELUTION IN CELL-AFFINITY CHROMATOGRAPHY5.8 NONSPECIFIC BINDING IN CELL SEPARATIONS; 5.9 SEPARATION OF RARE CELLS; 5.10 FLUORESCENCE-ACTIVATED CELL SORTING; 5.11 SORTING PARAMETERS; 5.12 OTHER SEPARATION TECHNIQUES AND CONSIDERATIONS; 5.13 CONCLUSION; REFERENCES; 6 Flow Cytometry: Cell Analysis in the Fast Lane; 6.1 INTRODUCTION; 6.2 THE CELL SAMPLE; 6.3 FLOW CYTOMETER FUNCTION; 6.4 OBTAINING OR FINDING A FLOW CYTOMETER; 6.5 USING FLOW CYTOMETERS; 6.6 SETTING UP A FLOW CYTOMETER FOR MULTI-COLOR STAINING; 6.7 ANALYZING FLOW CYTOMETRY DATA; 6.8 EXAMPLE FLOW-CYTOMETRY ASSAYS

6.9 NO-FLOW CYTOMETRY

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

Description based on metadata supplied by the publisher and other sources.

ISBN:

9786612482236

9781282482234

1282482238

9780470688441

0470688440

9780470688458

0470688459

OCLC:

587391030