Experimental glycoscience. Glycochemistry / N. Taniguchi ... [et al.] (eds.).

Format:

Book

Contributor:

Taniguchi, Naoyuki, 1942-

Language:

English

Subjects (All):

Glycoconjugates.

Oligosaccharides.

Physical Description:

1 online resource (259 p.)

Edition:

1st ed. 2008.

Place of Publication:

[Tokyo] ; New York : Springer, c2008.

Language Note:

English

Summary:

The aim of this book is to provide experimental protocols covering many aspects of glycobiology, glycotechnology, and chemistry: biochemistry, molecular and cellular biology, genetics, physiology, and medicine. The protocols are all self-contained descriptions of the equipment and reagents needed, followed by details of the experimental procedure. In the post-genomic era, glycobiology is coming of age because more than half of proteins are glycosylated and the importance of sugar chains in various fields of life science research cannot be disregarded. Many scientists had not entered this area because glycobiology and glycoscience used to be considered difficult fields. This book, therefore, is presented much like a cookbook which can help scientists in fields other than glycobiology and glycoscience carry out research more easily.

Contents:

Structural Analysis of Sugar Chains

Chemical Liberation of N-linked Oligosaccharides from Glycoproteins

Release of N-glycans by Enzymatic Methods

Release of O-glycans by Chemical Methods

Release of O-glycans by Enzymatic Methods

Ceramidase and Related Enzymes

Labeling of Oligosaccharides

Chemical Labeling of Sialyloligo/polymer

Separation of Oligosaccharides by 2D HPLC

Analysis of Oligosaccharides by Capillary Electrophoresis

Energy-Resolved Mass Spectrometry (ERMS) of Oligosaccharide

LC/MS of N-Linked Oligosaccharides

Convenient Structural Characterization of Intact Glycosphingolipids by MALDI-TOF Mass Spectrometry with Increased Laser Power and Cooling Gas Flow

Structural Analyses of Glycoconjugates by NMR

Sugar Chain Analysis by Enzymatic Digestion and 2D Mapping by HPLC

Analysis of Binding Sites of Sugar Chains by Methylation Analysis

Glycan Profiling

Monoclonal Antibody as a Clue to Structural Analysis of Bioactive Functional Glycoconjugates

Microsequencing of Functional Chondroitin Sulfate Oligosaccharides

Structural Characterization of PA-oligosaccharide Isomers Derived from Glycosphingolipids by MALDI-TOF Mass Spectrometry

Structural Analysis of Phospho-Glycosphingolipids in Lower Animals

Structural Analysis of Polysialic Acid

Milk Oligosaccharides: Structural Characterization and Future Aspects

An XML Description of Carbohydrate Structures

Construction of a Diagnostic Library for Glycans Using Multistage Tandem Mass Spectrometry (MS n )

A Method for Large-Scale Analysis for N-linked Glycoproteins by the Glycosylation Site-Specific Stable Isotope-Labeling and LC/MS Shotgun Technology

Mass Spectrometry of Glycopeptides

Development of MS Method for the Analysis of Sugar Peptide

Calculations for Saccharides by the Use of MS Data

Molecular Modeling of Oligosugar Structures

Prediction of Sugar-Binding Sites on Proteins

Frontal Affinity Chromatography: An Effective Analytical Tool for Protein-Sugar Interaction

Analyses of Sugar-Protein Interactions by NMR

Surface Plasmon Resonance and Sugar Chip Analysis for Sugar Chain-Protein Interactions

Analysis of Interactions Between Carbohydrates and Proteins Using Capillary Affinity Electrophoresis

Equilibrium Dialysis

Interaction Assay of Oligosaccharide with Protein Using Fluorescence Polarization (FP) and Fluorescence Correlation Spectroscopy (FCS)

Development of Neoglycoconjugate Probes and Detection of Lectins

Chemical Synthesis of Sugar Chains

Synthesis of Glycolipid and Its Application

Chemo-Enzymatic Synthesis of Glycoconjugates

Construction of O-Linked Glycopeptide Library Using Human Glycosyltransferases

Alteration of N-Linked Oligosaccharides

Stationary Solid-Phase Reaction (SSPR) for Oligosaccharide Synthesis

Sugar Chain Synthesis by the Use of Cell Functions

Glyco-Chemistry Cycle System Based on Glycosidases

Endoglycosidases (Glycosaminoglycans)

Oligosaccharide Synthesis Based on Combinatorial Chemistry and Labo Automation

Endoglycosidases (Glycoproteins)

Glycosaminoglycans

Recent Advances in the Production of Mammalian-Type Sugar Chains in Yeast

Solid-Phase Synthesis of Glycopeptides

Efficient Synthesis of Oligosaccharides and Synthesis of Pathogen-Associated Molecular Patterns for Their Biofunctional Studies

Sugar Polymers (Dendrimers and Pendant-Type Linear Polymers)

Rapid Synthesis of Oligosaccharides: Resin Capture-Release Strategy.

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

ISBN:

1-282-03839-7

9786612038396

4-431-77924-8

OCLC:

325001830