Human stem cell manual : a laboratory guide / edited by Jeanne F. Loring, Robin L. Wesselschmidt, Philip H. Schwartz.

Format:

Book

Contributor:

Loring, Jeanne Frances, 1950-

Schwartz, Philip H.

Wesselschmidt, Robin L.

Language:

English

Subjects (All):

Biology--Research--Methodology.

Biology.

Embryonic stem cells.

Stem cells--Laboratory manuals.

Stem cells.

Physical Description:

1 online resource (486 p.)

Edition:

1st ed.

Place of Publication:

Amsterdam ; Boston : Elsevier/Academic Press, 2007.

Language Note:

English

Summary:

Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical ""hands on"" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practi

Contents:

Front Cover; Human Stem Cell Manual; Copyright Page; Contents; Foreword by James Battey; Foreword by Jeannie Fontana; Preface by Jeanne Loring; Preface by Robin L. Wesselschmidt; Preface by Philip H. Schwartz; List of Contributors; Part I: Basic Methods in Stem Cell Culture; Chapter 1 Human Embryonic Stem Cell Culture; Chapter 2 Human Feeder Cells, Feeder-free, and Defined Culture Systems; Chapter 3 Mouse Embryonic Fibroblast Feeder Cells; Chapter 4 Cryopreservation of Human Embryonic Stem Cells; Part II: Characterization of Stem Cells; Chapter 5 Classical Cytogenetics: Karyotyping

Chapter 6 Spectral Karyotyping and Fluorescent in situ HybridizationChapter 7 Genotype and Epigenotype by Single Nucleotide Polymorphism (SNP) Analysis; Chapter 8 Flow Cytometric Analysis of Human Embryonic Stem Cells; Chapter 9 Immunocytochemical Analysis of Stem Cells; Chapter 10 Characterization of Stem Cells Using Reverse Transcriptase Polymerase Chain Reaction; Chapter 11 Gene Expression Profiling of Stem Cells by Microarray; Chapter 12 Generation of Human Embryonic Stem Cell-derived Teratomas; Chapter 13 Characterization of Human Embryonic Stem Cell-derived Teratomas

Part III: Differentiation of Human Embryonic CellsChapter 14 Embryoid Bodies and Neuroepithelial Development; Chapter 15 Motor Neuron and Dopamine Neuron Differentiation; Chapter 16 Oligodendrocyte Differentiation from Human Embryonic Stem Cells; Chapter 17 Cardiac Development of Human Embryonic Stem Cells; Chapter 18 Hematopoiesis from Human Embryonic Stem Cells; Part IV: Genetic Manipulation of Stem Cells; Chapter 19 Genetic Manipulation of Human Embryonic Stem Cells: Lentivirus Vectors; Chapter 20 Genetic Manipulation of Embryonic Stem Cells; Part V: Advanced Methods

Chapter 21 Derivation of Embryonic Stem Cells from Human BlastocystsChapter 22 Neural Progenitor Cell Culture; Chapter 23 Stem Cell Transplantation in the Brain; Chapter 24 In Vitro Fertilization; Chapter 25 Functional Characterization of Neurons; Part VI: Setting up a Laboratory; Chapter 26 Setting up a Facility for Human Embryonic Stem Cell Research; Part VII: Stem Cells and Society; Chapter 27 Intellectual Property: Owning the Stem Cell; Chapter 28 Ethical Concerns for Stem Cell Research; Chapter 29 Guidelines for Embryonic Stem Cell Research Oversight (ESCRO) Committees; Abbreviations

IndexA; B; C; D; E; F; G; H; I; K; L; M; N; O; P; Q; R; S; T; U; V; W; X; Y

Notes:

Description based upon print version of record.

Includes bibliographical references and index.

ISBN:

1-281-01881-3

9786611018818

0-08-054988-8

OCLC:

476087167