A laboratory guide to the mammalian embryo / edited by David K. Gardner, Michelle Lane, Andrew J. Watson.

Format:

Book

Contributor:

Gardner, David K., editor.

Lane, Michelle, editor.

Watson, Andrew J. (Andrew John), 1959- editor.

Series:

Oxford scholarship online.

Oxford scholarship online

Language:

English

Subjects (All):

Embryology, Experimental--Laboratory manuals.

Embryology, Human--Laboratory manuals.

Physical Description:

xiv, 394 p. : ill. (some col.).

Edition:

1st ed.

Place of Publication:

Oxford : Oxford University Press, 2023.

Language Note:

English

Summary:

Drawing from recent work with embryos from rodents, domestic animals, and primates, this practical guide describes the theory and protocols for performing biochemical, microscopic, and genetic techniques that have been developed and applied at the single-cell level. The techniques are the result of both new research and updates to established proce.

Contents:

Intro

Contents

Contributors

Abbreviations

1. Follicle Development in Vitro

2. Preparation of Gametes, in Vitro Maturation, in Vitro Fertilization, and Embryo Recovery and Transfer

3. Culture of the Mammalian Preimplantation Embryo

4. Assessment of Preimplantation Embryo Development and Viability

5. Micromanipulation of Gametes

6. Micromanipulation: Biopsy

7. Analysis of Intracellular Ions in Embryos: pH and Calcium

8. Assessment of Nutrient Uptake, Metabolite Production, and Enzyme Activity

9. Metabolic Pathway Activity

10. Confocal Imaging of Structural Molecules in Mammalian Gametes

11. Fluorescent in situ Hybridisation for Detection of Aneuploidy in Single Human Blastomeres for Preimplantation Genetic Diagnosis

12. Assessment of Ploidy, Telomere Length, and Telomerase Activity in Oocytes and Embryos

13. Microscale RNA Isolation from Mammalian Embryos

14. Relative mRNA Transcript Abundance in Early Embryos by Reverse Transcription-Polymerase Chain Reaction

15. Characterization of Novel Genes during Early Development by Application of Differential Display RT-PCR

16. Two-Dimensional Protein Gel Database Analysis of Embryos, Oocytes, and Oocyte-Associated Granulosa Cells

17. Antisense Disruption of Gene Expression in the Preimplantation Embryo

18. Gene Subtraction and Analysis

19. Analysis of Apoptosis in the Preimplantation Embryo

20. Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo

21. Human Embryonic Stem Cells

22. Spermatogonial Transplantation

23. Somatic Cell Nuclear Transfer

24. Cryobiology: Slow Freezing Vitrification of Embryos

Index.

Notes:

Previously issued in print: 2004.

Includes bibliographical references and index.

Derived record based on print version record and publisher information.

ISBN:

0-19-770144-2

1-4294-1517-7

9786610481729

9786610427888

1-280-48172-2

1-280-42788-4

0-19-803251-X

1-60256-787-5

OCLC:

935262024