Drosophila protocols / edited by William Sullivan, Michael Ashburner, R. Scott Hawley.

Format:

Book

Contributor:

Sullivan, William, 1957-

Ashburner, M.

Hawley, R. Scott.

Language:

English

Subjects (All):

Drosophila--Laboratory manuals.

Drosophila.

Physical Description:

xiv, 697 p. : ill. (some col.).

Place of Publication:

Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2000.

Contents:

Intro

Contents

Preface

Abbreviations

Preparation and Analysis of Drosophila Mitotic Chromosomes

In Situ Hybridization to Somatic Chromosomes

BrdU Labeling of Chromosomes

Analysis of Meiosis in Fixed and Live Oocytes by Light Microscopy

Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster

Preparation and Analysis of Polytene Chromosomes

In Situ Hybridization to Polytene Chromosomes

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

Fluorescent Analysis of Drosophila Embryos

Imaginal Discs

The Neuromuscular Junction

Histological Techniques for the Drosophila Eye Part I: Larva and Pupa

Histological Techniques for the Drosophila Eye Part II: Adult

Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy

Electrophysiological Approaches to the Neuromusculature

Functional Assays of the Peripheral and Central Nervous Systems

GFP and Other Reporters

Quantitative Microinjection of Drosophila Embryos

Targeted Disruption of Gene Function in Drosophila by RNA Interference

Drosophila Cell Culture and Transformation

Generating Antibodies against Drosophila Proteins

Photoactivated Gene Expression for Cell- fate Mapping and Cell Manipulation

Recovery of DNA Sequences Flanking P- element Insertions: Inverse PCR and Plasmid Rescue

GAL4- mediated Ectopic Gene Expression in Drosophila

Functional Cell Ablation

Preparation of DNA from E. coli Cells Containing Bacteriophage P1 Clones

Drosophila Microarrays: From Arrayer Construction to Hybridization

Using Drosophila Genome Databases

Preparation and Analysis of High- molecular- weight DNA in Drosophila

Culturing Large Populations of Drosophila for Protein Biochemistry*.

Preparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis

Preparation of Membrane Proteins from Drosophila Embryos

Preparing Cytoplasmic Extracts from Drosophila Embryos

Immunoblotting of Proteins from Single Drosophila Embryos

Laboratory Culture of Drosophila

Preparation of Larval and Adult Cuticles for Light Microscopy

Exposing Drosophila to Neuroactive Drugs

1989 Table of Contents from Drosophila: A Laboratory Manual

Anatomical Drawings of Drosophila

Solutions and Reagents

Cautions

Suppliers

Trademarks

Index.

Notes:

Includes bibliographical references and index.

OCLC:

928634304